Dye decolorization of magnetically retrievable formulation of laccase with amine-functionalized microparticles

Ferhan Korkmaz; Belma Nural Yaman; Serap Gedikli; Pınar Aytar Çelik; Murat Demirbilek; Ahmet Çabuk

doi:10.1016/j.mimet.2023.106755

Dye decolorization of magnetically retrievable formulation of laccase with amine-functionalized microparticles

J Microbiol Methods. 2023 Aug:211:106755. doi: 10.1016/j.mimet.2023.106755. Epub 2023 Jun 5.

Authors

Ferhan Korkmaz¹, Belma Nural Yaman², Serap Gedikli³, Pınar Aytar Çelik⁴, Murat Demirbilek⁵, Ahmet Çabuk⁶

Affiliations

¹ Department of Biology, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
² Department of Biomedical Engineering, Faculty of Engineering and Architect, Eskişehir Osmangazi University, Eskişehir, Turkey; Biotechnology and Biosafety Department, Graduate and Natural Applied Science, Eskişehir Osmangazi University, Eskişehir, Turkey. Electronic address: bnural@ogu.edu.tr.
³ Microbiota Biotechnology Industry and Trade Inc., Eskisehir Technology Development Zone Eskişehir, Turkey.
⁴ Biotechnology and Biosafety Department, Graduate and Natural Applied Science, Eskişehir Osmangazi University, Eskişehir, Turkey; Environmental Protection and Control Program, Eskişehir Osmangazi University, Eskişehir, Turkey.
⁵ Department of Biology, Polatli Faculty of Science and Letters, Ankara Haci Bayram Veli University, Ankara, Turkey.
⁶ Department of Biology, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey; Biotechnology and Biosafety Department, Graduate and Natural Applied Science, Eskişehir Osmangazi University, Eskişehir, Turkey.

PMID: 37285968
DOI: 10.1016/j.mimet.2023.106755

Abstract

Laccase is an important enzyme used in many industries because of its multi-substrate catalyst. New immobilization agents are excellent tools for enhancing the abilities of this enzyme. In this study, immobilization of laccase on silica microparticles with NH₂ (S-NH₂) surface modification to use in dye removal applications was aimed. The yield of immobilization by this method was found to be 93.93 ± 2.86% under optimum conditions. In addition, this newly created immobilized enzyme was adapted to a decolorization application with 87.56 ± 1.60% efficiency. Silica microparticles with NH₂ (S-NH₂) surface modification were used for laccase immobilization and this immobilized laccase had quite good potential. Besides, Random Amplified Polymorphic DNA (RAPD) analysis in evaluating the toxicity of the decolorization process was utilized. After amplification with two RAPD primers, decreased toxicity of dye in this study was observed. This study showed that RAPD analysis in toxicity testing could be accepted as an alternative and practical method that this approach will contribute to the literature in terms of providing fast and reliable results. The use of amine-modified surface silica microparticles for laccase immobilization and RAPD for toxicity testing is a crucial aspect of our investigation.

Keywords: Amine-modified surface; Immobilization; Magnetic particle; Microparticle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines*
Coloring Agents
Enzymes, Immobilized
Laccase* / genetics
Random Amplified Polymorphic DNA Technique
Silicon Dioxide

Substances

Laccase
Amines
Enzymes, Immobilized
Silicon Dioxide
Coloring Agents