Ocean Worlds beneath thick ice covers in our solar system, as well as subglacial lakes on Earth, may harbor biological systems. In both cases, thick ice covers (>100 s of meters) present significant barriers to access. Melt probes are emerging as tools for reaching and sampling these realms due to their small logistical footprint, ability to transport payloads, and ease of cleaning in the field. On Earth, glaciers are immured with various abundances of microorganisms and debris. The potential for bioloads to accumulate around and be dragged by a probe during descent has not previously been investigated. Due to the pristine nature of these environments, minimizing and understanding the risk of forward contamination and considering the potential of melt probes to act as instrument-induced special regions are essential. In this study, we examined the effect that two engineering descent strategies for melt probes have on the dragging of bioloads. We also tested the ability of a field cleaning protocol to rid a common contaminant, Bacillus. These tests were conducted in a synthetic ice block immured with bioloads using the Ice Diver melt probe. Our data suggest minimal dragging of bioloads by melt probes, but conclude that modifications for further minimization and use in special regions should be made.
Keywords: Clean access; Melt probe; Microbiology; Planetary protection; Special regions; Subglacial sampling.