Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos

Abstract

The development of single-cell multiomics has provided the ability to systematically investigate cellular diversity and heterogeneity in different biological systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to elucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.

Keywords: Blastocysts; Co-Seq; Embryos; Preimplantation development; Single-cell RNA sequencing; Single-cell multiomics; Small non-coding RNAs; Small-Seq; Smart-Seq2.