Ganoderenic acid D-loaded functionalized graphene oxide-based carrier for active targeting therapy of cervical carcinoma

Jiahui Lu; Anqiang Zhang; Fuming Zhang; Robert J Linhardt; Zhihui Zhu; Yanzi Yang; Tinghuang Zhang; Zhibin Lin; Su Zhang; Huajun Zhao; Peilong Sun

doi:10.1016/j.biopha.2023.114947

Ganoderenic acid D-loaded functionalized graphene oxide-based carrier for active targeting therapy of cervical carcinoma

Biomed Pharmacother. 2023 Aug:164:114947. doi: 10.1016/j.biopha.2023.114947. Epub 2023 Jun 2.

Authors

Jiahui Lu¹, Anqiang Zhang², Fuming Zhang³, Robert J Linhardt⁴, Zhihui Zhu⁵, Yanzi Yang⁵, Tinghuang Zhang⁵, Zhibin Lin⁶, Su Zhang⁷, Huajun Zhao⁸, Peilong Sun⁹

Affiliations

¹ College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 311402 Hangzhou, PR China; College of Food Science and Engineering, Zhejiang University of Technology, 310014 Hangzhou, PR China.
² College of Food Science and Engineering, Zhejiang University of Technology, 310014 Hangzhou, PR China.
³ Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 12180 Troy, NY, USA.
⁴ Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 12180 Troy, NY, USA; Departments of Chemistry and Chemical Biology and Biomedical Engineering, Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 12180 Troy, NY, USA.
⁵ College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 311402 Hangzhou, PR China.
⁶ State Key Laboratory of Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical Sciences, Peking University, 100191 Beijing, PR China.
⁷ Zhejiang Provincial Rural Industrial Development Co., Ltd, 310000, Hangzhou, PR China.
⁸ College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 311402 Hangzhou, PR China. Electronic address: zhj@zcmu.edu.cn.
⁹ College of Food Science and Engineering, Zhejiang University of Technology, 310014 Hangzhou, PR China. Electronic address: sun_pl@zjut.edu.cn.

PMID: 37269813
DOI: 10.1016/j.biopha.2023.114947

Abstract

Ganoderenic acid D (GAD), extracted from the Chinese herb Ganoderma lucidum, was loaded onto a graphene oxide-polyethylene glycol-anti-epidermal growth factor receptor (GO-PEG-EGFR) carrier to develop a targeting antitumor nanocomposite (GO-PEG@GAD). The carrier was fabricated from PEG and anti-EGFR aptamer modified GO. Targeting was mediated by the grafted anti-EGFR aptamer, which targets the membrane of HeLa cells. Physicochemical properties were characterized by transmission electron microscopy, dynamic light scattering, X-ray powder diffraction, and Fourier transform infrared spectroscopy. High loading content (77.3 % ± 1.08 %) and encapsulation efficiency (89.1 % ± 2.11 %) were achieved. Drug release continued for approximately 100 h. The targeting effect both in vitro and in vivo was confirmed by confocal laser scanning microscopy (CLSM) and imaging analysis system. The mass of the subcutaneous implanted tumor was significantly decreased by 27.27 ± 1.23 % after treatment with GO-PEG@GAD compared with the negative control group. Moreover, the in vivo anti-cervical carcinoma activity of this medicine was due to activation of the intrinsic mitochondrial pathway.

Keywords: Active targeting antitumor; Cervical carcinoma; Ganoderenic acid D.

MeSH terms

Antineoplastic Agents* / chemistry
Antineoplastic Agents* / pharmacology
Antineoplastic Agents* / therapeutic use
Drug Carriers / chemistry
Graphite* / chemistry
HeLa Cells
Humans
Polyethylene Glycols / chemistry
Spectroscopy, Fourier Transform Infrared

Substances

graphene oxide
Antineoplastic Agents
Graphite
Drug Carriers
Polyethylene Glycols