Lateral flow immunoassays for antigens, antibodies and haptens detection

Ge Li; Qingmei Li; Xun Wang; Xiao Liu; Yuhang Zhang; Rui Li; Junqing Guo; Gaiping Zhang

doi:10.1016/j.ijbiomac.2023.125186

Lateral flow immunoassays for antigens, antibodies and haptens detection

Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125186. doi: 10.1016/j.ijbiomac.2023.125186. Epub 2023 Jun 1.

Authors

Ge Li¹, Qingmei Li², Xun Wang³, Xiao Liu⁴, Yuhang Zhang³, Rui Li², Junqing Guo⁵, Gaiping Zhang⁶

Affiliations

¹ College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
² Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
³ College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
⁴ Henan Medical College, Zhengzhou 451191, China.
⁵ Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Electronic address: guojunqing2008@gmail.com.
⁶ College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China; Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address: zhanggaip@126.com.

Abstract

Lateral flow immunoassay (LFIA) is widely used as a rapid point-of-care testing (POCT) technique in food safety, veterinary and clinical detection on account of the accessible, fast and low-cost characteristics. After the outbreak of the coronavirus disease 2019 (COVID-19), different types of LFIAs have attracted considerable interest because of their ability of providing immediate diagnosis directly to users, thereby effectively controlling the outbreak. Based on the introduction of the principles and key components of LFIAs, this review focuses on the major detection formats of LFIAs for antigens, antibodies and haptens. With the rapid innovation of detection technologies, new trends of novel labels, multiplex and digital assays are increasingly integrated with LFIAs. Therefore, this review will also introduce the development of new trends of LFIAs as well as its future perspectives.

Keywords: Antibodies; Antigens; Detection format; Haptens; Lateral flow immunoassays; New trends.

Publication types

Review

MeSH terms

Antibodies
Antigens
COVID-19* / diagnosis
Haptens*
Humans
Immunoassay / methods

Substances

Haptens
Antibodies
Antigens