Ratiometric fluorescent detection of carbendazim in foods based on metallic nanoclusters self-assembled nanocomplex

Xiujin Chen; Guowen Li; Xin Yue; Chifang Peng; Jun Wang

doi:10.1016/j.foodchem.2023.136478

Ratiometric fluorescent detection of carbendazim in foods based on metallic nanoclusters self-assembled nanocomplex

Food Chem. 2023 Oct 30:424:136478. doi: 10.1016/j.foodchem.2023.136478. Epub 2023 May 27.

Authors

Xiujin Chen¹, Guowen Li², Xin Yue², Chifang Peng³, Jun Wang⁴

Affiliations

¹ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China. Electronic address: chenxiujin9610@126.com.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China. Electronic address: pcf@jiangnan.edu.cn.
⁴ Shandong Institute for Food and Drug Control, Xinluo Road 2749, Jinan, Shandong 250101, China. Electronic address: sdzjywj@163.com.

PMID: 37267653
DOI: 10.1016/j.foodchem.2023.136478

Abstract

Indicator replacement assay combining with fluorescence resonance energy transfer (FRET) effect has attractive performance in sensing small molecules, however, there wasn't application in pesticide molecule sensing reported so far. In this work, we prepared a nanocomplex (NCP), AuAgNCs-CD, through self-assembly of gold nanoclusters (AuNCs), silver nanoclusters (AgNCs) and carboxymethyl-β-cyclodextrin (CM-β-CD) by one-step method. The emission of AuNCs was significantly enhance. It was found that FRET between the AuAgNCs-CD and rhodamine B (RhB) existed after AuAgNCs-CD combined with RhB. And carbendazim (CBZ) could induce anti-FRET effect through competing with RhB and binding to AuAgNCs-CD. Thus, this phenomenon was utilized to develop a ratiometric fluorescent detection of CBA. This method was applied in food sample detection and reliable results were obtained. Due to high sensitivity, rapidness and good selectivity, this ratiometric fluorescent method was expected to hold high application potential in monitoring CBZ in foods.

Keywords: Aggregation induced emission enhancement; Carbendazim; FRET; Indicator replacement assay; Metal nanoclusters.

MeSH terms

Benzimidazoles
Fluorescence Resonance Energy Transfer / methods
Fluorescent Dyes / chemistry
Gold / chemistry
Metal Nanoparticles* / chemistry
Spectrometry, Fluorescence

Substances

carbendazim
Fluorescent Dyes
Benzimidazoles
Gold