Qualitative and quantitative analysis of four benzimidazole residues in food by surface-enhanced Raman spectroscopy combined with chemometrics

Tianyao Wang; Chuangjie Xie; Qian You; Xingguo Tian; Xiaoyan Xu

doi:10.1016/j.foodchem.2023.136479

Qualitative and quantitative analysis of four benzimidazole residues in food by surface-enhanced Raman spectroscopy combined with chemometrics

Food Chem. 2023 Oct 30:424:136479. doi: 10.1016/j.foodchem.2023.136479. Epub 2023 May 27.

Authors

Tianyao Wang¹, Chuangjie Xie¹, Qian You¹, Xingguo Tian², Xiaoyan Xu³

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: xingguot@scau.edu.cn.
³ Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: yanzixu_2001@scau.edu.cn.

PMID: 37263093
DOI: 10.1016/j.foodchem.2023.136479

Abstract

In this study, surface-enhanced Raman spectroscopy (SERS) combined with chemometric methods were developed for qualitative and quantitative analysis of four benzimidazole (BMZs) residues in corn. Sulfhydryl functionalized Fe₃O₄@SiO₂@Ag-SH magnetic SERS substrates were prepared to obtain the SERS spectra of four BMZs for chemometric analysis. The partial least squares regression discrimination analysis (PLS-DA) model performed best, with a recall rate upwards 99.17%, and could successfully distinguish four BMZs. Under the support vector machine regression (SVR) model, the detection limits of carbendazim, benomyl, thiophanate-methyl and thiabendazole were 0.055 mg/L, 0.056 mg/L, 0.067 mg/L and 0.093 mg/L, respectively; the average recovery was in the range of 85.6%-107.5%. Furthermore, the method verified by HPLC, and the results showed that there was no significant difference between two methods (p > 0.05). Therefore, the strategy based on SERS coupling chemometrics can be served as a promising tool for rapid determination of BMZs residues in food.

Keywords: Benzimidazole pesticides; Chemometrics; Simultaneous detection; Sulfhydryl functionalization; Surface-enhanced Raman spectroscopy.

MeSH terms

Benzimidazoles / analysis
Chemometrics
Metal Nanoparticles* / chemistry
Silicon Dioxide
Spectrum Analysis, Raman* / methods
Thiabendazole / analysis

Substances

Silicon Dioxide
Benzimidazoles
Thiabendazole