Targeted Quantification of Amino Acids by Dansylation

Yuanyuan Liu; Haoqing Chen; Dylan Dodd

doi:10.1007/978-1-0716-3247-5_6

Targeted Quantification of Amino Acids by Dansylation

Methods Mol Biol. 2023:2675:65-76. doi: 10.1007/978-1-0716-3247-5_6.

Authors

Yuanyuan Liu¹, Haoqing Chen¹, Dylan Dodd^{2

3}

Affiliations

¹ Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
² Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. ddodd2@stanford.edu.
³ Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA. ddodd2@stanford.edu.

PMID: 37258756
DOI: 10.1007/978-1-0716-3247-5_6

Abstract

Quantification of amino acids in biological samples is a critical tool for studying metabolism. Although many methods for amino acid analysis exist, important considerations include ease of sample preparation, dynamic range, reproducibility, instrument availability, and throughput. Here, we present a simple, rapid, and robust method for the analysis of amino acids by chemical derivatization and liquid chromatography-mass spectrometry (LC-MS). We provide a detailed protocol for the analysis of 20 proteinogenic amino acids in biological samples which will enable straightforward implementation on modern LC-MS instruments.

Keywords: Amino acid analysis; Dansylation; Liquid chromatography-mass spectrometry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids* / chemistry
Chromatography, Liquid / methods
Mass Spectrometry
Reproducibility of Results
Specimen Handling*

Substances

Amino Acids

Abstract

Publication types

MeSH terms

Substances

Grants and funding