Resurrection of Leucobryumscalare Müll.Hal. ex M.Fleisch. (Bryophyta, Leucobryaceae) based on phylogenetic and morphometric evidence

Patsakorn Tiwutanon; Kasidis Chaiyasut; H Thorsten Lumbsch; Ekaphan Kraichak

doi:10.3897/phytokeys.222.98990

Resurrection of Leucobryumscalare Müll.Hal. ex M.Fleisch. (Bryophyta, Leucobryaceae) based on phylogenetic and morphometric evidence

PhytoKeys. 2023 Mar 20:222:27-47. doi: 10.3897/phytokeys.222.98990. eCollection 2023.

Authors

Patsakorn Tiwutanon¹, Kasidis Chaiyasut², H Thorsten Lumbsch³, Ekaphan Kraichak¹

Affiliations

¹ Department of Botany, Faculty of Science, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
² Science Division, Mahidol University International College, Mahidol University, 999 Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
³ Science & Education, The Field Museum, 1400 South Lake Shore Drive, Chicago, Illinois, USA.

Abstract

Leucobryumscalare was described in 1904 but its taxonomic status has been disputed, being reduced to a variety of Leucobryumaduncum or synonymized with Leucobryumaduncum. The taxonomic confusion of this taxon has remained unresolved. Hence, we revisited the taxonomic status of the taxon using phylogenetic and morphometric approaches. A total of 27 samples from Leucobryumaduncumvar.aduncum and Leucobryumaduncumvar.scalare were used to generate data from four markers, including ITS1, ITS2, atpB-rbcL spacer, and trnL-trnF. The concatenated dataset was used to reconstruct a phylogenetic tree. Both qualitative and quantitative morphological characters were measured and analyzed with Principal Component Analysis (PCA) and PERMANOVA. The results showed that the two taxa are closely related but they are reciprocally monophyletic. Both qualitative and quantitative characters could also separate Leucobryumaduncumvar.scalare from Leucobryumaduncumvar.aduncum as shown with PCA and PERMANOVA. We propose the resurrection of the species rank for Leucobryumscalare as separate from Leucobryumaduncum. This work highlights the need for a more thorough revision of Leucobryum to clarify the actual level of diversity in this genus.

Keywords: Bryophytes; classification; mosses; revision; tropical biodiversity.

Patsakorn Tiwutanon, Kasidis Chaiyasut, H. Thorsten Lumbsch, Ekaphan Kraichak.

Grants and funding

the Office of the Ministry of Higher Education, Science, Research and Innovation, and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021 and the Graduate Scholarship of the Faculty of Science, Kasetsart University of the Year 2019.