Genome-wide characterization, phylogenetic and expression analysis of ABCG gene subfamily in cucumber (Cucumis sativus L.)

Qi Yuan; Jing Zhang; Wanlu Zhang; Jingtao Nie

doi:10.3389/fpls.2023.1178710

Genome-wide characterization, phylogenetic and expression analysis of ABCG gene subfamily in cucumber (Cucumis sativus L.)

Front Plant Sci. 2023 May 11:14:1178710. doi: 10.3389/fpls.2023.1178710. eCollection 2023.

Authors

Qi Yuan^{1

2

3}, Jing Zhang^{1

2

3}, Wanlu Zhang¹, Jingtao Nie^{1

2

3}

Affiliations

¹ College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang, China.
² Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang, China.
³ Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang, China.

Abstract

The ABCG is the largest subfamily of the ABC family with extensive functions, and only a few members have been identified in detail. However, more and more studies have shown that the members of this family are very important and are involved in many life processes such as plant development and response to various stresses. Cucumber is an important vegetable crops around the world. The cucumber development is essential for its production and quality. Meanwhile, various stresses have caused serious losses of cucumber. However, the ABCG genes were not well characterized and functioned in cucumber. In this study, the cucumber CsABCG gene family were identified and characterized, and their evolutionary relationship and functions were analyzed. The cis-acting elements and expression analysis showed that they played important role in development and responding to various biotic and abiotic stresses in cucumber. Phylogenetic analysis, sequence alignment and MEME (Multiple Em for Motif Elicitation) analysis indicated that the functions of ABCG proteins in different plants are evolutionarily conserved. Collinear analysis revealed that the ABCG gene family was highly conserved during the evolution. In addition, the potential binding sites of the CsABCG genes targeted by miRNA were predicted. These results will lay a foundation for further research on the function of the CsABCG genes in cucumber.

Keywords: CsABCG; bioinformatic character; cucumber; gene expression; microRNA; stress responses.

Grants and funding

This research was funded by National Natural Science Foundation of China (No. 31701915), Zhejiang Province Public Welfare Technology Application Research Project (No. LGN19C150007), and a Start-Up Found from Zhejiang A&F University (2017FR006).