Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Huibin Zhang; Jinglin Wang; Fan Xie; Yangguang Liu; Mengyao Qiu; Zheng Han; Yueyun Ding; Xianrui Zheng; Zongjun Yin; Xiaodong Zhang

doi:10.3389/fcell.2023.1169745

Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Front Cell Dev Biol. 2023 May 5:11:1169745. doi: 10.3389/fcell.2023.1169745. eCollection 2023.

Authors

Huibin Zhang^{1

2}, Jinglin Wang^{1

2}, Fan Xie^{1

2}, Yangguang Liu^{1

2}, Mengyao Qiu^{1

2}, Zheng Han^{1

2}, Yueyun Ding^{1

2}, Xianrui Zheng^{1

2}, Zongjun Yin^{1

2}, Xiaodong Zhang^{1

2}

Affiliations

¹ College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
² Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, Hefei, China.

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that play a crucial role in the complex and dynamic network that regulates the apoptosis of porcine ovarian granulosa cells (POGCs). Resveratrol (RSV) is a nonflavonoid polyphenol compound that is involved in follicular development and ovulation. In previous study, we established a model of RSV treatment of POGCs, confirming the regulatory effect of RSV in POGCs. To investigate the miRNA-level effects of RSV on POGCs to reveal differentially expressed miRNAs, a control group (n = 3, 0 μM RSV group), a low RSV group (n = 3, 50 μM RSV group), and a high RSV group (n = 3, 100 μM RSV group) were created for small RNA-seq. In total, 113 differentially expressed miRNAs (DE-miRNAs) were identified, and a RT-qPCR analysis showed a correlation with the sequencing data. Functional annotation analysis revealed that DE-miRNAs in the LOW vs. CON group may be involved in cell development, proliferation, and apoptosis. In the HIGH vs. CON group, RSV functions were associated with metabolic processes and responses to stimuli, while the pathways were related to PI3K24, Akt, Wnt, and apoptosis. In addition, we constructed miRNA-mRNA networks related to Apoptosis and Metabolism. Then, ssc-miR-34a and ssc-miR-143-5p were selected as key miRNAs. In conclusion, this study provided an improved understanding of effects of RSV on POGCs apoptosis through the miRNA modulations. The results suggest that RSV may promote POGCs apoptosis by stimulating the miRNA expressions and provided a better understanding of the role of miRNAs combined with RSV in ovarian granulosa cell development in pigs.

Keywords: apoptosis; miRNA; ovary; pig; resveratrol.

Grants and funding

This work was supported by the National Key Research and Development Program of China (2021YFD1301200), the National Natural Science Foundation of China (31972531), the Cooperative Innovation Project of Anhui provincial universities (GXXT-2021-055), the Joint Research Project on Local Pig breeding in Anhui Province (340000211260001000431), and the Major special science and technology project of Anhui Province (202103a06020013).