Bioimprinted lipase-catalyzed synthesis of medium- and long-chain structured lipids rich in docosahexaenoic acid for infant formula

Xiaoqiang Zou; Heng Su; Fengcheng Zhang; Hongjiang Zhang; Yeliaman Yeerbolati; Xiuli Xu; Zhonghao Chao; Lei Zheng; Bangzhi Jiang

doi:10.1016/j.foodchem.2023.136450

Bioimprinted lipase-catalyzed synthesis of medium- and long-chain structured lipids rich in docosahexaenoic acid for infant formula

Food Chem. 2023 Oct 30:424:136450. doi: 10.1016/j.foodchem.2023.136450. Epub 2023 May 24.

Authors

Xiaoqiang Zou¹, Heng Su², Fengcheng Zhang³, Hongjiang Zhang³, Yeliaman Yeerbolati³, Xiuli Xu³, Zhonghao Chao³, Lei Zheng³, Bangzhi Jiang³

Affiliations

¹ State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China. Electronic address: xiaoqiangzou@163.com.
² State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China. Electronic address: 1012210128@stu.jiangnan.edu.cn.
³ State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.

PMID: 37247604
DOI: 10.1016/j.foodchem.2023.136450

Abstract

Medium- and long-chain structured lipids (MLSLs) rich in docosahexaenoic acid (DHA) were obtained in shorter reaction time by acidolysis of single-cell oil (DHASCO) from Schizochytrium sp. with caprylic acid (CA) using a lipase bioimprinted with fatty acids as a catalyst. The conditions for preparation of the bioimprinted lipase for the acidolysis reaction were firstly optimized and the activity of the obtained lipase was 2.17 times higher than that of the non-bioimprinted. The bioimprinted lipase was then used as a catalyst and the reaction conditions were optimized. Under the optimal conditions, the equilibrium could be achieved in 4 h, and the total and sn-1,3 CA contents in the product were 29.18% and 42.34%, respectively, and the total and sn-2 DHA contents were 46.26% and 70.12%, respectively. Such MLSLs rich in sn-1,3 CA and sn-2 DHA are beneficial for DHA absorption, and thus have potential for use in infant formula.

Keywords: Acidolysis; Bioavailability; Bioimprinting; Docosahexaenoic acid; Infant formula; Medium- and long-chain structured lipids.

MeSH terms

Catalysis
Docosahexaenoic Acids*
Fatty Acids
Humans
Infant
Infant Formula
Lipase*
Triglycerides

Substances

Docosahexaenoic Acids
Lipase
Fatty Acids
octanoic acid
Triglycerides