A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

Sonal Fande; Sangam Srikanth; Jayapiriya U S; Khairunnisa Amreen; Satish Kumar Dubey; Arshad Javed; Sanket Goel

doi:10.1016/j.xpro.2023.102327

A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

STAR Protoc. 2023 May 25;4(2):102327. doi: 10.1016/j.xpro.2023.102327. Online ahead of print.

Authors

Sonal Fande¹, Sangam Srikanth², Jayapiriya U S¹, Khairunnisa Amreen¹, Satish Kumar Dubey², Arshad Javed², Sanket Goel³

Affiliations

¹ Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronic (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India.
² Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronic (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India.
³ Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronic (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India. Electronic address: sgoel@hyderabad.bits-pilani.ac.in.

Abstract

Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device with its integrations. We then detail the electrochemical detection of bacteria using a microfluidic fuel cell. The laser-induced graphene heater provides the temperature for the bacterial culture, and metabolic activity is recognized using a bacterial fuel cell. Please see Srikanth et al.¹ for comprehensive information on the application and execution of this protocol.

Keywords: Biotechnology and bioengineering; Material sciences.