Protocol for early warning of Diaporthe infection in kiwifruit soft rot by plasmonic dimer-enhanced Raman spectroscopy

Hao Jiang; Ting Yu; Min Miao; Honglin Liu

doi:10.1016/j.xpro.2023.102339

Protocol for early warning of Diaporthe infection in kiwifruit soft rot by plasmonic dimer-enhanced Raman spectroscopy

STAR Protoc. 2023 May 26;4(2):102339. doi: 10.1016/j.xpro.2023.102339. Online ahead of print.

Authors

Hao Jiang¹, Ting Yu¹, Min Miao¹, Honglin Liu²

Affiliations

¹ China Light Industry Key Laboratory of Meat Microbial Control and Utilization, School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, P.R. China.
² China Light Industry Key Laboratory of Meat Microbial Control and Utilization, School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, P.R. China. Electronic address: liuhonglin@mail.ustc.edu.cn.

Abstract

The pathogens of the Diaporthe genus are now considered to be the dominant pathogens of kiwifruit soft rot. Here, we present a protocol to construct nanoprobes for the Diaporthe genus and detect changes of surface-enhanced Raman spectroscopy for samples collected from infected kiwifruit. We describe steps for synthesis of gold nanoparticles, DNA extraction from kiwifruit, and construction of nanoprobes. We then detail classification of nanoparticles with different aggregation states through dark-field microscope (DFM) picture analysis using Fiji-ImageJ software. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).¹.

Keywords: Chemistry; Plant sciences.