Allicin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection In Vitro and Alleviates Inflammatory Responses

Jingbo Hu; Chenxi Li; Yanyang Zhou; Jingjing Ding; Xiangdong Li; Yanhua Li

doi:10.3390/v15051050

Allicin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection In Vitro and Alleviates Inflammatory Responses

Viruses. 2023 Apr 25;15(5):1050. doi: 10.3390/v15051050.

Authors

Jingbo Hu¹, Chenxi Li^{1

2

3}, Yanyang Zhou¹, Jingjing Ding¹, Xiangdong Li^{1

2

3}, Yanhua Li^{1

2

3}

Affiliations

¹ College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
² Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China.
³ Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens to the swine industry worldwide over the past three decades. No approved effective antiviral drug is available to control this virus. The antiviral effects of allicin (diallyl thiosulfinate) on many human and animal viruses have been documented. However, the antiviral effect of allicin on PRRSV infection remains unknown. In this study, we found that allicin exhibited an inhibitory effect on HP-PRRSV and NADC30-like PRRSV in a dose-dependent manner by interfering with viral entry, replication, and assembly. Furthermore, allicin alleviated the expression of pro-inflammatory cytokines (IFN-β, IL-6, and TNFα) induced by PRRSV infection. The pro-inflammatory signaling pathways, TNF signaling pathway and MAPK signaling pathway, up-regulated by PRRSV infection were restored by allicin treatment. Taken together, these results demonstrate that allicin has antiviral activity against PRRSV and ameliorates inflammatory responses induced by PRRSV infection, suggesting that allicin is a promising drug candidate for anti-PRRSV therapy in vivo.

Keywords: allicin; anti-inflammatory; porcine reproductive and respiratory syndrome virus; viral entry; viral replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiviral Agents / pharmacology
Cell Line
Humans
Porcine Reproductive and Respiratory Syndrome* / drug therapy
Porcine respiratory and reproductive syndrome virus*
Swine
Virus Replication

Substances

allicin
Antiviral Agents

Grants and funding

This research was supported by the National Natural Science Foundation of China (Grant No. 32072833), the 111 Project D18007, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).