Hepatic Transcriptomics Reveals Reduced Lipogenesis in High-Salt Diet Mice

Jing Xu; Fei Mao; Yan Lu; Tiemin Liu; Xiaoying Li; Yao Li

doi:10.3390/genes14050966

Hepatic Transcriptomics Reveals Reduced Lipogenesis in High-Salt Diet Mice

Genes (Basel). 2023 Apr 24;14(5):966. doi: 10.3390/genes14050966.

Authors

Jing Xu¹, Fei Mao¹, Yan Lu², Tiemin Liu³, Xiaoying Li¹, Yao Li⁴

Affiliations

¹ Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
² Institute of Metabolism and Regenerative Medicine, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
³ School of Life Sciences, Fudan University, Shanghai 200032, China.
⁴ Department of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, China.

Abstract

It has been demonstrated that a high salt diet (HSD) increases the risk of cardiovascular disease and metabolic dysfunction. In particular, the impact and molecular mechanisms of long-term HSD on hepatic metabolism remain largely unknown. To identify differentially expressed genes (DEGs) affecting the metabolism of liver tissues from HSD and control groups, a transcriptome analysis of liver tissues was performed in this study. As a result of the transcriptome analysis, the expression of genes related to lipid and steroid biosynthesis (such as Fasn, Scd1, and Cyp7a1) was significantly reduced in the livers of HSD mice. Additionally, several gene ontology (GO) terms have been identified as associated with metabolic processes in the liver, including the lipid metabolic process (GO: 0006629) and the steroid metabolic process (GO: 0008202). An additional quantitative RT-qPCR analysis was conducted to confirm six down-regulated genes and two up-regulated genes. Our findings provide a theoretical basis for further investigation of HSD-induced metabolic disorders.

Keywords: fatty acid profile; high-salt diet; liver; metabolism; transcriptomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diet
Gene Expression Profiling
Lipids
Lipogenesis* / genetics
Liver / metabolism
Mice
Steroids / metabolism
Transcriptome*

Substances

Lipids
Steroids

Grants and funding

This work is supported by the National Natural Science Foundation of China (No. 81820108008, No. 81900727, No. 82070887) and the Shanghai Jiaotong University Foundation (No. shklab202012).