Japanese Flounder pol-miR-155 Is Involved in Edwardsiella tarda Infection via ATG3

Zhanwei Zhang; Xiaolu Guan

doi:10.3390/genes14050958

Japanese Flounder pol-miR-155 Is Involved in Edwardsiella tarda Infection via ATG3

Genes (Basel). 2023 Apr 22;14(5):958. doi: 10.3390/genes14050958.

Authors

Zhanwei Zhang^{1

2}, Xiaolu Guan¹

Affiliations

¹ CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
² College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.

Abstract

MicroRNAs (miRNAs) are small RNA molecules that function in the post-transcriptionally regulation of the expression of diverse genes, including those involved in immune defense. Edwardsiella tarda can infect a broad range of hosts and cause severe disease in aquatic species, including Japanese flounder (Paralichthys olivaceus). In this study, we examined the regulation mechanism of a flounder miRNA, pol-miR-155, during the infection of E. tarda. Pol-miR-155 was identified to target flounder ATG3. Overexpression of pol-miR-155 or knockdown of ATG3 expression suppressed autophagy and promoted the intracellular replication of E. tarda in flounder cells. Overexpression of pol-miR-155 activated the NF-κB signaling pathway and further promoted the expression of downstream immune related genes of interleukin (IL)-6 and IL-8. These results unraveled the regulatory effect of pol-miR-155 in autophagy and in E. tarda infection.

Keywords: MicroRNA; Paralichthys olivaceus; autophagy; bacterial infection; immune regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Edwardsiella tarda / genetics
Enterobacteriaceae Infections* / genetics
Enterobacteriaceae Infections* / veterinary
Fish Diseases* / genetics
Flounder*
MicroRNAs* / genetics
MicroRNAs* / metabolism

Substances

MicroRNAs

Grants and funding

This work was supported by the grants from the National Key Research and Development Program of China (2018YFD0900505), the grants of the National Natural Science Foundation of China (31730100), the project ZR2021QC124 supported by Shandong Provincial Natural Science Foundation and the Taishan Scholar Program of Shandong Province.