New Anti-Hypoxic Metabolites from Co-Culture of Marine-Derived Fungi Aspergillus carneus KMM 4638 and Amphichorda sp. KMM 4639

Biomolecules. 2023 Apr 25;13(5):741. doi: 10.3390/biom13050741.

Abstract

The KMM 4639 strain was identified as Amphichorda sp. based on two molecular genetic markers: ITS and β-tubulin regions. Chemical investigation of co-culture marine-derived fungi Amphichorda sp. KMM 4639 and Aspergillus carneus KMM 4638 led to the identification of five new quinazolinone alkaloids felicarnezolines A-E (1-5), a new highly oxygenated chromene derivative oxirapentyn M (6) and five previously reported related compounds. Their structures were established using spectroscopic methods and by comparison with related known compounds. The isolated compounds showed low cytotoxicity against human prostate and breast cancer cells but felicarnezoline B (2) protected rat cardiomyocytes H9c2 and human neuroblastoma SH-SY5Y cells against CoCl2-induced damage.

Keywords: ITS; antioxidants; co-culture; cobalt chloride (II); cytoprotection; marine-derived fungi; oxyrapentyn; phylogeny; quinazoline alkaloid; secondary metabolites; β-tubulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coculture Techniques
  • Fungi / chemistry
  • Humans
  • Hypocreales*
  • Molecular Structure
  • Neuroblastoma*
  • Rats

Supplementary concepts

  • Aspergillus carneus

Grants and funding

This research was funded by a grant from the Ministry of Science and Higher Education of Russian Federation 15.BRK.21.0004 (Contract No. 075-15-2021-1052).