Assessing the impact of rainwater harvesting infrastructure and gardening trends on microbial indicator organism presence in harvested rainwater and garden soils

J Appl Microbiol. 2023 Jun 1;134(6):lxad110. doi: 10.1093/jambio/lxad110.

Abstract

Aim: To assess the microbial water quality of harvested rainwater infrastructure used to supplement household water uses for homegrown produce.

Methods and results: Using a co-created community science methodology, between 2017 and 2020, a total of 587 harvested rainwater samples and 147 garden soil samples irrigated with harvested rainwater were collected from four Arizona communities and analyzed for coliform, Escherichia coli, and/or Salmonella. Participants also completed a home description survey regarding their home and surrounding area, water harvesting infrastructure, and gardening habits.

Conclusion: Chi-Square tests revealed that the quality of harvested rainwater is affected by proximity to a waste disposal or incineration facility, animal presence, cistern treatment, and cistern age (P < 0.05), while soil samples were associated with community (P < 0.05). Coliform and E. coli concentrations in both sample types were greater in the monsoon season.

Keywords: E. coli; Salmonella; citizen science; community science; garden soil; rainwater harvesting.

MeSH terms

  • Escherichia coli*
  • Gardening*
  • Gardens
  • Rain
  • Soil
  • Water Microbiology
  • Water Supply

Substances

  • Soil