Transcription factor-mediated programming of stem cell fate

Yun Zhao; Xi Wang; Kai Wang

doi:10.1016/j.tcb.2023.05.004

Transcription factor-mediated programming of stem cell fate

Trends Cell Biol. 2023 Aug;33(8):621-624. doi: 10.1016/j.tcb.2023.05.004. Epub 2023 May 24.

Authors

Yun Zhao¹, Xi Wang², Kai Wang³

Affiliations

¹ Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China.
² Clinical Stem Cell Research Center, Peking University Third Hospital, Beijing 100191, China.
³ Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China; Clinical Stem Cell Research Center, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Metabolic Disorder Related Cardiovascular Disease, Beijing 100050, China; State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Beijing 100037, China. Electronic address: kai.wang88@pku.edu.cn.

PMID: 37236901
DOI: 10.1016/j.tcb.2023.05.004

Abstract

Direct activation of transcription factors (TFs) in human pluripotent stem cells (hPSCs) allows more rapid and efficient conversion of cell fates compared with classical approaches. Here, we summarize recent TF screening studies and established forward programming methods for different cell types, and highlight their current limitations and future perspectives.

Keywords: differentiation; forward programming; stem cells; transcription factors.

MeSH terms

Cell Differentiation
Gene Expression Regulation
Humans
Pluripotent Stem Cells*
Transcription Factors* / metabolism

Substances

Transcription Factors