Viral vectors as carriers of genome-editing reagents

Muhammad Jawad Akbar Awan; Afzal Akram; Imran Amin; Shahid Mansoor

doi:10.1016/j.tplants.2023.05.010

Viral vectors as carriers of genome-editing reagents

Trends Plant Sci. 2023 Sep;28(9):981-983. doi: 10.1016/j.tplants.2023.05.010. Epub 2023 May 24.

Authors

Muhammad Jawad Akbar Awan¹, Afzal Akram¹, Imran Amin¹, Shahid Mansoor²

Affiliations

¹ Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.
² Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan; International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. Electronic address: shahidmansoor7@gmail.com.

PMID: 37236860
DOI: 10.1016/j.tplants.2023.05.010

Abstract

The presence of a transgene in the genome of plants is a regulatory challenge. Recently, Liu et al. reported an engineered tomato spotted wilt virus (TSWV) that can carry large clustered regularly interspaced palindromic repeats (CRISPR)/Cas reagents for targeted genome editing in various crops without the integration of the transgene into the genome.

Keywords: CRISPR-Cas; CRISPR-Cas12a; genome editing; heritable mutagenesis; mobile RNA; non-transgenic; viral vector.

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MeSH terms

CRISPR-Cas Systems* / genetics
Crops, Agricultural / genetics
Gene Editing*