Surgical method for critical sized cranial defects in rat cranium

Sedat Odabas; Burak Derkuş; İbrahim Vargel; Altughan Cahit Vural

doi:10.1016/j.mex.2023.102208

Surgical method for critical sized cranial defects in rat cranium

MethodsX. 2023 May 4:10:102208. doi: 10.1016/j.mex.2023.102208. eCollection 2023.

Authors

Sedat Odabas^{1

2}, Burak Derkuş^{2

3}, İbrahim Vargel⁴, Altughan Cahit Vural⁵

Affiliations

¹ Ankara University, Faculty of Science, Department of Chemistry, Biomaterials and Tissue Engineering Laboratory (bteLAB), Turkey.
² Interdisciplinary Research Unit for Advanced Materials (INTRAM), Ankara University, Ankara, Turkey.
³ Ankara University, Faculty of Science, Department of Chemistry, Stem Cell Research Laboratory (SCRLab), Ankara, Turkey.
⁴ Hacettepe University, Faculty of Medicine, Department of Plastic Reconstructive and Aesthetic Surgery, Ankara, Turkey.
⁵ Harbiye Mah. Abdi İpekci Cad. 3/3 Şişli, İstanbul, Turkey.

Abstract

Cranial tissue models are a widely used model to show the bone repair and the regeneration ability of candidate biomaterials for tissue engineering purposes. Until now, efficacy studies of different biomaterials for calvarial defect bone regeneration have been reported, generally in small animal models. This paper offers a versatile, reliable, and reproducible surgical method for creating a critical-sized cranial defect in rats including critical steps and tried-and-tested tips. The method proposed here,•Shows a general procedure for in vivo cranial models.•Provide an insight to restore bone tissue repair that may be used in combination with several tissue engineering strategies•Is a crucial technique that may guide in vivo bone tissue engineering.

Keywords: Bone tissue engineering; Cranium; Creating a critical sized bone defect in rat model; Critical size.