2,4-Dinitrotoluene (2,4-DNT) exposure induces liver developmental damage and perturbs lipid metabolism and oxygen transport gene expression in zebrafish (Danio rerio)

Abstract

2,4-Dinitrotoluene (2, 4-DNT) is a common environmental pollutant. The toxic effect on mammals of 2,4-DNT has been well studied, but its toxicity on aquatic organisms is little known. In this study, 126 healthy female zebrafish (Danio rerio) were exposed to different concentrations of 2,4-DNT (0, 2, 4, 8, 12 and 16 mg/L) to determine 96-h semi-lethal concentrations (LC₅₀). And then, 90 female zebrafish were exposed to 0, 2, 4 and 8 mg/L 2,4-DNT for 5 days to study liver toxicity. Exposed zebrafish developed hypoxia features, such as floating head and breathing rapidly, and then died. 96-h LC₅₀ of 2,4-DNT in zebrafish was 9.36 mg/L. Histological data revealed that 2,4-DNT severely damaged the liver tissues, following with the round nucleus, dense interstitial tissue, dense arranged hepatocyte cords and more inflammatory cells. Additionally, the further result showed that the lower levels of lipid transport and metabolism (apoα2, mtp, ppar-α and acox) were noticed. But, exposed to 2,4-DNT for 5 days significantly upregulated the expression levels of genes involved in respiration (hif1a, tfa and ho1, p < 0.05). These results indicated that 2,4-DNT exposure disturbed lipid transport and metabolism and oxygen supply in zebrafish, which could contribute to severe damage in liver and death.

Keywords: 2,4-dinitrotoluene; Lipid metabolism; Liver; Oxygen transport; Zebrafish.