In vitro and in vivo assessment of structural integrity for HPCD complex@Liposome nanocomposites from ocular surface to the posterior segment of the eye

Jinhui Lu; Xiaomeng Zhu; Meng Zhang; Xunchan Jiang; Wei Guo; Feng Jiang; Feng Cao

doi:10.1016/j.carbpol.2023.120960

In vitro and in vivo assessment of structural integrity for HPCD complex@Liposome nanocomposites from ocular surface to the posterior segment of the eye

Carbohydr Polym. 2023 Sep 1:315:120960. doi: 10.1016/j.carbpol.2023.120960. Epub 2023 Apr 27.

Authors

Jinhui Lu¹, Xiaomeng Zhu², Meng Zhang², Xunchan Jiang², Wei Guo³, Feng Jiang⁴, Feng Cao⁵

Affiliations

¹ Department of Pharmaceutical, School of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing, 210009, China; Department of Dizal Pharmaceutical Co. Ltd., 199 Liangjing Rd, Pudong District, Shanghai 201203, China.
² Department of Pharmaceutical, School of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing, 210009, China.
³ Jiangsu Key Laboratory of Druggability of Biopharmaceuticals and State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: guowei1205@cpu.edu.cn.
⁴ Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China. Electronic address: jiangfeng@cpu.edu.cn.
⁵ Department of Pharmaceutical, School of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing, 210009, China. Electronic address: cpufengc@cpu.edu.cn.

PMID: 37230631
DOI: 10.1016/j.carbpol.2023.120960

Abstract

Investigating the structural integrity of carriers in transit from ocular surface to ocular posterior segment is essential for an efficient topical drug delivery system. In this study, dual-carrier hydroxypropyl-β-cyclodextrin complex@Liposome (HPCD@Lip) nanocomposites were developed for the efficient delivery of dexamethasone. Förster Resonance Energy Transfer with near-infrared I fluorescent dyes and in vivo imaging system were used to investigate the structural integrity of HPCD@Lip nanocomposites after crossing Human conjunctival epithelial cells (HConEpiC) monolayer and in ocular tissues. The structural integrity of inner HPCD complexes was monitored for the first time. The results suggested that 23.1 ± 6.4 % of nanocomposites and 41.2 ± 4.3 % of HPCD complexes could cross HConEpiC monolayer with an intact structure at 1 h. 15.3 ± 8.4 % of intact nanocomposites could reach at least sclera and 22.9 ± 1.2 % of intact HPCD complexes could reach choroid-retina after 60 min in vivo, which showed that the dual-carrier drug delivery system could successfully deliver intact cyclodextrin complexes to ocular posterior segment. In conclusion, in vivo assessment of structural integrity of nanocarriers is greatly significant for guiding the rational design, higher drug delivery efficiency and clinical transformation for topical drug delivery system to the posterior segment of the eye.

Keywords: Cyclodextrin complexes; Dual-carrier; Förster Resonance Energy Transfer (FRET); Liposome; Posterior segment of the eye; Structural integrity investigation.

MeSH terms

2-Hydroxypropyl-beta-cyclodextrin
Drug Delivery Systems / methods
Excipients
Humans
Liposomes*
Nanocomposites* / chemistry
Retina

Substances

Liposomes
2-Hydroxypropyl-beta-cyclodextrin
Excipients