Objectives: Unprecedented challenges in hospital care were imposed in response to the COVID-19 pandemic due to the highly contagious disease. Healthcare services adjusted for working with additional personal protection equipment and hygiene measures while attending to a large number of critically ill patients. In this study, we aimed to determine burnout rates and preferred interventions among healthcare staff, including nurses and physicians, at Bnai-Zion Medical Center during the COVID-19 pandemic. Methods: The Copenhagen Burnout Inventory questionnaire was administered to 185 cross-sectional volunteer participants from the nursing and medical staff between June and August 2020, when Israel experienced its second surge of COVID-19. Results: We found a statistically significant correlation between work-related and personal burnout. COVID-19 ward staff had greater burnout levels than the rest of our institution's personnel. Most highly burned-out healthcare workers were interested in intervention therapy. Conclusions: Dealing with burnout is imperative to improve the well-being of our hospital staff and ensure the best possible performance. Nursing management should consider first-line responders' stressful conditions to be alleviated through support programs.