The role of silver nanoparticles effects in the homeostasis of metals in soybean cultivation through qualitative and quantitative laser ablation bioimaging

Katherine Chacón-Madrid; Danielle da Silva Francischini; Marco Aurélio Zezzi Arruda

doi:10.1016/j.jtemb.2023.127207

The role of silver nanoparticles effects in the homeostasis of metals in soybean cultivation through qualitative and quantitative laser ablation bioimaging

J Trace Elem Med Biol. 2023 Sep:79:127207. doi: 10.1016/j.jtemb.2023.127207. Epub 2023 May 18.

Authors

Katherine Chacón-Madrid¹, Danielle da Silva Francischini¹, Marco Aurélio Zezzi Arruda²

Affiliations

¹ Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
² Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil. Electronic address: zezzi@unicamp.br.

PMID: 37224744
DOI: 10.1016/j.jtemb.2023.127207

Abstract

Background: Nanoparticles (NPs) are currently found in the world in the form of natural colloids and volcanic ash, as well as in anthropogenic sources, such as nanofertilizers; however, in the literature, there is still a lack of toxicological evidence, risk assessment, and regulations about the use and environmental impact of NPs in the agroindustrial system. Therefore, the aim of this work was to evaluate alterations caused by the presence of AgNPs during the development of soybean plants.

Methods: The BRS232 non-transgenic (NT) soybean plant and 8473RR (T_RR) and INTACTA RR2 PRO (T_Intacta) transgenic soybean plants were irrigated for 18 days under controlled conditions with deionized water (control), AgNPs, and AgNO₃. The isotopes ¹⁰⁷Ag⁺, ⁵⁵Mn⁺, ⁵⁷Fe⁺, ⁶³Cu⁺, and ⁶⁴Zn⁺ were mapped in leaves, using ¹³C⁺ as an internal standard (IS), and carried out using a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) technique with a Nd:YAG (213 nm) laser source in the imagagin mode using the LA-iMageS software and also Mathlab.

Results: Leaf images showed a low Ag translocation, indicated by the basal signal of this ion. Additionally, the presence of Ag in the ionic form and as NPs altered the homeostasis of ¹¹²Cd⁺, ⁶⁴Zn⁺, ⁵⁵Mn⁺, ⁶³Cu⁺, and ⁵⁷Fe⁺ in different ways. Quantitative image analysis was performed for Cu.

Conclusion: The behavior of T_RR and T_Intacta plants was different in the presence of ionic silver or AgNPs, confirming that the metabolism of these two plants, despite both being transgenic, are different. Through the images, it was observed that the response of plants was different in the face of the same stress conditions during their development.

Keywords: LA-ICP-MS; Metals homeostasis; Quantitative imaging; Silver nanoparticles; Soybean; Transgenic plants.

MeSH terms

Glycine max / chemistry
Homeostasis
Laser Therapy* / methods
Metal Nanoparticles* / chemistry
Plants
Silver / metabolism

Substances

Silver