Development of Biocellulose Sheet Incorporating Aloe vera Gel Extract for Diabetic Wound Healing

Atchariya Yosboonruang; Preeyawass Phimnuan; Swanya Yakaew; Bovornlak Oonkhanond; Anchalee Rawangkan; Sukunya Ross; Gareth Ross; Jarupa Viyoch

doi:10.1021/acsomega.3c00372

Development of Biocellulose Sheet Incorporating Aloe vera Gel Extract for Diabetic Wound Healing

ACS Omega. 2023 May 3;8(19):16824-16832. doi: 10.1021/acsomega.3c00372. eCollection 2023 May 16.

Authors

Atchariya Yosboonruang¹, Preeyawass Phimnuan², Swanya Yakaew², Bovornlak Oonkhanond³, Anchalee Rawangkan¹, Sukunya Ross⁴, Gareth Ross⁴, Jarupa Viyoch²

Affiliations

¹ Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
² Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
³ Globrick Refractories Company Limited, Nonthaburi 11000, Thailand.
⁴ Department of Chemistry, Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.

Abstract

In this study, a biocellulose (BC) sheet containing Aloe vera gel extract (AE) was developed for application in healing chronic wounds, such as diabetic wounds. The BC sheet was produced by Acetobacter xylinum and then lyophilized to obtain dried sheets. A. vera gel was extracted by precipitation in 35% ammonium sulfate, lyophilized, dried, and incorporated into the BC sheet. The protein content of the AE was 12.32 ± 3.4% w/w, with a molecular weight of ∼20 kDa. The release of TNF-α from lipopolysaccharide-induced RAW264.7 cells was reduced by treatment with AE in a dose-dependent manner. The physicochemical and biological properties of the developed sheet were investigated. Morphological examination of the BC/AE sheet using scanning electron microscopy revealed the 3D construction of nanofibrils, which showed high porosity. The BC/AE sheet exhibited water absorption at 74%, and the release of proteins in the AE reached 97.23% at 4 h. The BC sheet incorporated with proteins in the AE at 283.78 ± 7.7 μg/cm² can promote the wound healing in streptozotocin-induced diabetic rats. The recovering skin in diabetic wounds treated with the BC/AE sheet exhibited a normal cell arrangement without fibrosis, as revealed by histological staining. The research findings indicate that the BC/AE sheet has potential for applications in wound dressings.