Genomic and 16S metabarcoding data of Holothuria tubulosa Gmelin, 1791

Maria Kyritsi; George Tsiolas; Sofia Michailidou; Konstantinos Koukaras; Anagnostis Argiriou

doi:10.1016/j.dib.2023.109171

Genomic and 16S metabarcoding data of Holothuria tubulosa Gmelin, 1791

Data Brief. 2023 Apr 22:48:109171. doi: 10.1016/j.dib.2023.109171. eCollection 2023 Jun.

Authors

Maria Kyritsi¹, George Tsiolas¹, Sofia Michailidou¹, Konstantinos Koukaras¹, Anagnostis Argiriou^{1

2}

Affiliations

¹ Institute of Applied Biosciences / CERTH, P.O. Box 60361, Thermi, Thessaloniki 57001, Greece.
² Department of Food Science and Nutrition, University of the Aegean, Lemnos 81400, Greece.

Abstract

Holothuria tubulosa Gmelin, 1791 is an edible sea cucumber species widespread in the Mediterranean Sea with ecological and increasing economic importance. Genome data of holothurian species is limited and the availability of genomic data resources is crucial in understanding their biology and adaptability mechanisms. This dataset presents the raw genome sequence data of H. tubulosa sequenced on an Illumina NextSeq 2000 platform. Genome size estimation was performed based on k-mer frequency approach. Additionally, the bacterial microbiome in the stomach and intestine of H. tubulosa collected from the Strymonian Gulf (North Aegean Sea, Greece) through 16S rRNA amplicon metabarcoding sequencing is reported. Sequencing was performed on an Illumina MiSeq platform. Analysis was conducted using the QIIME2 software package, the DADA2 algorithm and a trained classifier for taxonomy assignment. The datasets presented in this work serve as valuable resources for a comprehensive investigation of H. tubulosa at the genome level and for comparative genomics and echinoderms gut microbial studies.

Keywords: 16S rRNA; Echinoderms; Gastrointestinal tract microbiome; Holothuria tubulosa; Illumina; Metagenomics; Sea cucumber; Whole genome sequencing.