The Use of Single-Cell RNA-Sequencing and Spatial Transcriptomics in Understanding the Pathogenesis and Treatment of Skin Diseases

Aubrey E Houser; Abiha Kazmi; Arjun K Nair; Andrew L Ji

doi:10.1016/j.xjidi.2023.100198

The Use of Single-Cell RNA-Sequencing and Spatial Transcriptomics in Understanding the Pathogenesis and Treatment of Skin Diseases

JID Innov. 2023 Mar 22;3(4):100198. doi: 10.1016/j.xjidi.2023.100198. eCollection 2023 Jul.

Authors

Aubrey E Houser^{1

2}, Abiha Kazmi^{1

2}, Arjun K Nair^{1

2}, Andrew L Ji^{1

3

2

4}

Affiliations

¹ Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
² Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
³ Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
⁴ Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Abstract

The development of multiomic profiling tools has rapidly expanded in recent years, along with their use in profiling skin tissues in various contexts, including dermatologic diseases. Among these tools, single-cell RNA-sequencing (scRNA-seq) and spatial transcriptomics (ST) have emerged as widely adopted and powerful assays for elucidating key cellular components and their spatial arrangement within skin disease. In this paper, we review the recent biological insights gained from the use of scRNA-seq and ST and the advantages of combining both for profiling skin diseases, including aberrant wound healing, inflammatory skin diseases, and cancer. We discuss the role of scRNA-seq and ST in improving skin disease treatments and moving toward the goal of achieving precision medicine in dermatology, whereby patients can be optimally matched to treatments that maximize therapeutic response.

Publication types

Review

Grants and funding

K08 CA263187/CA/NCI NIH HHS/United States