A benzo BODIPY based fluorescent probe for selective visualization of hypochlorous acid in living cells and zebrafish

Abstract

Hypochlorous acid (HOCl) is an essential endogenous reactive oxygen species in biological systems, playing a critical role in various physiological processes. Real-time monitoring of HOCl concentration in living organisms is essential for understanding its biological functions and pathological roles. In this study, we developed a novel fluorescent probe based on benzobodipy, BBDP, for rapid and sensitive detection of HOCl in aqueous solutions. The probe exhibited a significant fluorescence turn-on response to HOCl based on its specific oxidation reaction towards diphenylphosphine, with high selectivity, instantaneous response (less than 10 s), and low detection limit (21.6 nM). Furthermore, bioimaging results illustrated that the probe could be applied for real-time fluorescence imaging of HOCl in live cells and zebrafish. The development of BBDP may provide a new tool for exploring the biological functions of HOCl and its pathological roles in diseases.

Keywords: Fluorescent probe; Hypochlorous acid; Living cell imaging; Zebrafish imaging.