Use of brewer's residual yeast for production of bacterial nanocellulose with Gluconacetobacter hansenii

Gabriela Martins de Paiva; Letícia Fernanda de Melo; Fernanda Palladino Pedroso; Patrícia da Luz Mesquita; Edson Romano Nucci; Igor José Boggione Santos

doi:10.1016/j.ijbiomac.2023.124897

Use of brewer's residual yeast for production of bacterial nanocellulose with Gluconacetobacter hansenii

Int J Biol Macromol. 2023 Jul 1;242(Pt 3):124897. doi: 10.1016/j.ijbiomac.2023.124897. Epub 2023 May 15.

Authors

Gabriela Martins de Paiva¹, Letícia Fernanda de Melo², Fernanda Palladino Pedroso³, Patrícia da Luz Mesquita¹, Edson Romano Nucci⁴, Igor José Boggione Santos⁵

Affiliations

¹ Graduate Program in Chemical Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36420-000 Ouro Branco, MG, Brazil.
² Graduate Program in Bioprocess Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36420-000 Ouro Branco, MG, Brazil.
³ Institute of Biological Sciences, Federal University of Minas Gerais, Pres. Antônio Carlos Avenue, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
⁴ Graduate Program in Chemical Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36420-000 Ouro Branco, MG, Brazil; Departament of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36497-899 Ouro Branco, MG, Brazil.
⁵ Graduate Program in Chemical Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36420-000 Ouro Branco, MG, Brazil; Departament of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João del-Rei, Campus Alto Paraopeba, MG 443, km 7, 36497-899 Ouro Branco, MG, Brazil. Electronic address: igorboggione@ufsj.edu.br.

PMID: 37196713
DOI: 10.1016/j.ijbiomac.2023.124897

Abstract

Bacterial nanocellulose (BNC) has attained elevated interest due to its versatile structure and high resistance characteristics. Accordingly, efforts have been made in order to reduce its production costs, such as the employment of its by-products as a nutrient broth to yield the microorganism. Residual brewer's yeast is an excellent recourse, due to its high nutritional value and availability. Therefore, research which aimed to contribute to the development of a low cost, efficient and biosustainable technology for BNC production with Gluconacetobacter hansenii was carried out. BNC was obtained from residual brewer's yeast hydrolysate at pH 7.0 and five days of incubation at 30 °C in static culture. The hydrolysate was characterized by the amount of sugars, fatty acids, total proteins and ash content. Subsequently, BNC obtained was characterized in terms of yield, carbon conversion ratio, hydrodynamic size, crystallinity, morphology, Fourier-transform infrared spectroscopy, and surface analysis. Residual brewer's yeast hydrolysate proved to be efficient in BNC production via gluconeogenesis with consumption of alanine, threonine and glycerol, obtaining 1.9 times the yield of the chemically defined broth adopted as standard. Additionally, properties observed in the obtained BNC were equal to those obtained from conventional chemical medium. The research contributed to bacterial nanocellulose production using by-products from the brewing industry.

Keywords: Alternative substrate; BNC; DCCR; Fermentation; Hydrolysate; Waste beer yeast.

MeSH terms

Acetobacteraceae*
Fermentation
Saccharomyces cerevisiae* / metabolism

Supplementary concepts

Komagataeibacter hansenii