Sohlh1 and Lhx8 are prominent biomarkers to estimate the primordial follicle pool in mice

Li Liu; Biting Liu; Lian Wang; Caixia Li; Yang Zhou; Jihui Zhu; Jinye Ding; Shupeng Liu; Zhongping Cheng

doi:10.1186/s12958-023-01097-3

Sohlh1 and Lhx8 are prominent biomarkers to estimate the primordial follicle pool in mice

Reprod Biol Endocrinol. 2023 May 16;21(1):46. doi: 10.1186/s12958-023-01097-3.

Authors

Li Liu^{1

2}, Biting Liu¹, Lian Wang^{1

2}, Caixia Li^{1

2}, Yang Zhou^{1

2}, Jihui Zhu^{1

2}, Jinye Ding¹, Shupeng Liu^{3

4}, Zhongping Cheng^{5

6}

Affiliations

¹ Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.
² Institute of Gynecological Minimally Invasive Medicine, School of Medicine, Tongji University, Shanghai, 200072, China.
³ Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China. lshup@tongji.edu.cn.
⁴ Institute of Gynecological Minimally Invasive Medicine, School of Medicine, Tongji University, Shanghai, 200072, China. lshup@tongji.edu.cn.
⁵ Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China. mdcheng18@tongji.edu.cn.
⁶ Institute of Gynecological Minimally Invasive Medicine, School of Medicine, Tongji University, Shanghai, 200072, China. mdcheng18@tongji.edu.cn.

Abstract

Efficient evaluation of the primordial follicle pool (PFP) of mammalian models is an essential subject in biomedical research relating to ovarian physiology and pathogenesis. Our recent study has identified a gene signature including Sohlh1, Nobox, Lhx8, Tbpl2, Stk31, Padi6, and Vrtn strongly correlated with ovarian reserve by using bioinformatics analysis. Aimed to investigate the validity of these candidate biomarkers for evaluating the PFP, we utilized an OR comparison model to decode the relationship between the numbers of PFP and candidate biomarkers in the present study. Our results suggest that these biomarkers Sohlh1, Nobox, Lhx8, Tbpl2, Stk31, Padi6, and Vrtn possess independent potential to evaluate the number of the PFP. And the combination of Sohlh1 and Lhx8 can be used as the optimal biomarkers for rapid assessment of the PFP in the murine ovary. Our findings provide a new perspective for evaluating the PFP of the ovary in animal studies and the clinic.

Keywords: Biomarkers; Clinical assessment; Lhx8; Primordial follicle pool; Sohlh1.

MeSH terms

Animals
Basic Helix-Loop-Helix Transcription Factors / genetics
Female
Mice
Ovarian Follicle* / physiology
Ovary
Transcription Factors* / genetics

Substances

Basic Helix-Loop-Helix Transcription Factors
Sohlh1 protein, mouse
Transcription Factors
LIM homeobox protein 8