A core-shell structure ratiometric fluorescent probe based on carbon dots and Tb3+ for the detection of anthrax biomarker

Hui Heng; Deming Ma; Qingyang Gu; Jinyan Li; Haibo Jin; Ping Shen; Jiaxin Wei; Ziwei Wang

doi:10.1016/j.saa.2023.122793

A core-shell structure ratiometric fluorescent probe based on carbon dots and Tb³⁺ for the detection of anthrax biomarker

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15:299:122793. doi: 10.1016/j.saa.2023.122793. Epub 2023 May 3.

Authors

Hui Heng¹, Deming Ma², Qingyang Gu³, Jinyan Li¹, Haibo Jin¹, Ping Shen¹, Jiaxin Wei¹, Ziwei Wang¹

Affiliations

¹ College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China; Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, Beijing 102617, China.
² College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.
³ College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China; Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, Beijing 102617, China. Electronic address: guqingyang@bipt.edu.cn.

PMID: 37187145
DOI: 10.1016/j.saa.2023.122793

Abstract

A novel core-shell structure ratiometric fluorescent probe was developed, which can selectively and sensitively detect 2,6-dipicolinic acid (DPA) as an anthrax biomarker. Carbon dots (CDs) was embedded into SiO₂ nanoparticles, which was acted as an internal reference signal. Tb³⁺ with green emission was connected to the carboxyl functionalized SiO₂, which was acted as a responsive signal. With the addition of DPA, the emission of CDs at 340 nm was unchanged, while the fluorescence of Tb³⁺ at 544 nm was enhanced by the antenna effect. In the concentration range of 0.1-2 μM, the fluorescence intensity ratio of I₅₄₄/I₃₄₀ showed a good linear relationship with the concentration of DPA, and the limit of detection (LOD) was 10.2 nM. In addition, the dual-emission probe showed an obvious fluorescence color change from colourless to green with increasing DPA under UV light, which enabled visual detection.

Keywords: Anthrax biomarker; Carbon dots; Core–shell structure; Ratiometric fluorescent probe; Tb(3+).

MeSH terms

Anthrax* / diagnosis
Biomarkers
Carbon / chemistry
Fluorescent Dyes / chemistry
Humans
Quantum Dots* / chemistry
Silicon Dioxide / chemistry

Substances

Fluorescent Dyes
Carbon
Silicon Dioxide
Biomarkers