Construction of a CCL20-centered circadian-signature based prognostic model in cervical cancer

Yuchong Yu; Yao Liu; Yuhong Li; Xiaoming Yang; Mi Han; Qiong Fan

doi:10.1186/s12935-023-02926-6

Construction of a CCL20-centered circadian-signature based prognostic model in cervical cancer

Cancer Cell Int. 2023 May 15;23(1):92. doi: 10.1186/s12935-023-02926-6.

Authors

Yuchong Yu^{1

2

3}, Yao Liu^{1

2

3}, Yuhong Li^{1

2

3}, Xiaoming Yang^{1

2

3}, Mi Han^{4

5

6}, Qiong Fan^{7

8

9}

Affiliations

¹ Department of Gynecologic Oncology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
² Shanghai Municipal Key Clinical Specialty of Gynecologic Oncology, Shanghai, China.
³ Shanghai Key Laboratory of Embryo Original Diseases Affifiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁴ Department of Gynecologic Oncology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. hanmi880905@139.com.
⁵ Shanghai Municipal Key Clinical Specialty of Gynecologic Oncology, Shanghai, China. hanmi880905@139.com.
⁶ Shanghai Key Laboratory of Embryo Original Diseases Affifiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. hanmi880905@139.com.
⁷ Department of Gynecologic Oncology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. qiongfanjiao@163.com.
⁸ Shanghai Municipal Key Clinical Specialty of Gynecologic Oncology, Shanghai, China. qiongfanjiao@163.com.
⁹ Shanghai Key Laboratory of Embryo Original Diseases Affifiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. qiongfanjiao@163.com.

Abstract

Background: Rather low vaccination rates for Human papillomavirus (HPV) and pre-existing cervical cancer patients with limited therapeutic strategies ask for more precise prognostic model development. On the other side, the clinical significance of circadian clock signatures in cervical cancer lacks investigation.

Methods: Subtypes classification based upon eight circadian clock core genes were implemented in TCGA-CESC through k-means clustering methods. Afterwards, KEGG, GO and GSEA analysis were conducted upon differentially expressed genes (DEGs) between high and low-risk groups, and tumor microenvironment (TME) investigation by CIBERSORT and ESTIMATE. Furthermore, a prognostic model was developed by cox and lasso regression methods, and verified in GSE44001 by time-dependent receiver-operating characteristic curve (ROC) analysis. Lastly, FISH and IHC were used for validation of CCL20 expression in patients' specimens and U14 subcutaneous tumor models were built for TME composition.

Results: We successfully classified cervical patients into high-risk and low-risk groups based upon circadian-oscillation-signatures. Afterwards, we built a prognostic risk model composed of GJB2, CCL20 and KRT24 with excellent predictive value on patients' overall survival (OS). We then proposed metabolism unbalance, especially for glycolysis, and immune related pathways to be major enriched signatures between the high-risk and low-risk groups. Then, we proposed an 'immune-desert'-like suppressive myeloid cells infiltration pattern in high-risk group TME and verified its resistance to immunotherapies. Finally, CCL20 was proved positively correlated with real-world patients' stages and induced significant less CD8⁺ T cells and more M2 macrophages infiltration in mouse model.

Conclusions: We unraveled a prognostic risk model based upon circadian oscillation and verified its solidity. Specifically, we unveiled distinct TME immune signatures in high-risk groups.

Keywords: Bioinformatics; Cervical cancer; Circadian clock; Immunology; Prognostic model.