Multi-b-value diffusion stretched-exponential model parameters correlate with MIB-1 and CD34 expression in Glioma patients, an intraoperative MR-navigated, biopsy-based histopathologic study

Junlong Wang; Hua Zhang; Xuefei Dang; Wenting Rui; Haixia Cheng; Jing Wang; Yong Zhang; Tianming Qiu; Zhenwei Yao; Hanqiu Liu; Haopeng Pang; Yan Ren

doi:10.3389/fonc.2023.1104610

Multi-b-value diffusion stretched-exponential model parameters correlate with MIB-1 and CD34 expression in Glioma patients, an intraoperative MR-navigated, biopsy-based histopathologic study

Front Oncol. 2023 Apr 26:13:1104610. doi: 10.3389/fonc.2023.1104610. eCollection 2023.

Authors

Junlong Wang¹, Hua Zhang², Xuefei Dang³, Wenting Rui¹, Haixia Cheng⁴, Jing Wang¹, Yong Zhang⁵, Tianming Qiu⁶, Zhenwei Yao¹, Hanqiu Liu¹, Haopeng Pang^{7

8}, Yan Ren¹

Affiliations

¹ Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.
² Department of Radiology, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.
³ Department of Oncology, Minhang Branch of Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China.
⁴ Department of Neuropathology, Huashan Hospital, Fudan University, Shanghai, China.
⁵ Department of Magnetic Resonance Research, General Electric Healthcare, Shanghai, China.
⁶ Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
⁷ Minimally Invasive Therapy Center, Shanghai Cancer Center, Fudan University, Shanghai, China.
⁸ Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, China.

Abstract

Background: To understand the pathological correlations of multi-b-value diffusion-weighted imaging (MDWI) stretched-exponential model (SEM) parameters of α and diffusion distribution index (DDC) in patients with glioma. SEM parameters, as promising biomarkers, played an important role in histologically grading gliomas.

Methods: Biopsy specimens were grouped as high-grade glioma (HGG) or low-grade glioma (LGG). MDWI-SEM parametric mapping of DDC₁₅₀₀, α₁₅₀₀ fitted by 15 b-values (0-1,500 sec/mm²)and DDC₅₀₀₀ and α₅₀₀₀ fitted by 22 b-values (0-5,000 sec/mm²) were matched with pathological samples (stained by MIB-1 and CD34) by coregistered localized biopsies, and all SEM parameters were correlated with these pathological indices pMIB-1(percentage of MIB-1 expression positive rate) and CD34-MVD (CD34 expression positive microvascular density for each specimen). The two-tailed Spearman's correlation was calculated for pathological indexes and SEM parameters, as well as WHO grades and SEM parameters.

Results: MDWI-derived α₁₅₀₀ negatively correlated with CD34-MVD in both LGG (6 specimens) and HGG (26 specimens) (r=-0.437, P =0.012). MDWI-derived DDC₁₅₀₀ and DDC₅₀₀₀ negatively correlated with MIB-1 expression in all glioma patients (P<0.05). WHO grades negatively correlated with α₁₅₀₀(r=-0.485; P=0.005) and α₅₀₀₀(r=-0.395; P=0.025).

Conclusions: SEM-derived DDC and α are significant in histologically grading gliomas, DDC may indicate the proliferative ability, and CD34 stained microvascular perfusion may be an important determinant of water diffusion inhomogeneity α in glioma.

Keywords: biopsy; diffusion magnetic resonance imaging; glioma; intraoperative navigation; stretched exponential model.

Grants and funding

This work was supported by Shanghai Science and Technology Commission [No. 18411967300] and National Natural Science Foundation of China [No. 82001761 and No. 82102114].