Antibiotic resistance associated lactic acid cross tolerance in Shiga-toxin producing E. coli

Ikechukwu Oguadinma; Abhinav Mishra; Govindaraj Dev Kumar

doi:10.3389/fmicb.2023.1059144

Antibiotic resistance associated lactic acid cross tolerance in Shiga-toxin producing E. coli

Front Microbiol. 2023 Apr 26:14:1059144. doi: 10.3389/fmicb.2023.1059144. eCollection 2023.

Authors

Ikechukwu Oguadinma¹, Abhinav Mishra², Govindaraj Dev Kumar¹

Affiliations

¹ Center for Food Safety, The University of Georgia, Griffin, GA, United States.
² Department of Food Science & Technology, The University of Georgia, Athens, GA, United States.

Abstract

Introduction: The occurrence of antibiotic resistant (ABR) bacteria in foods is a growing public health challenge. We evaluated sanitizer cross-tolerance among ABR Escherichia coli (E. coli) O157:H7 and non-O157:H7 Shiga-toxin producing E. coli (STEC) serogroups. Sanitizer tolerance in STEC could be a public health concern as mitigation strategies against the pathogen might be compromised.

Methods: Resistance to ampicillin and streptomycin were evolved in E. coli serogroups: O157:H7 (H1730, and ATCC 43895), O121:H19 and O26:H11. Resistance to antibiotics was evolved chromosomally through incremental exposure to ampicillin (amp C) and streptomycin (strep C). Transformation using a plasmid was performed to confer resistance to ampicillin to generate amp P strep C.

Results: The minimum inhibitory concentration (MIC) of lactic acid for all strains evaluated was 0.375% v/v. Analysis of bacterial growth parameters in tryptic soy broth amended with 0.0625% v/v, 0.125% v/v, and 0.25% v/v (subMIC) lactic acid indicated that growth correlated positively with the lag phase duration, and negatively with both the maximum growth rate and change in population density for all strains evaluated except for the highly tolerant variant- O157:H7 amp P strep C. Strains O121 NR (non-ABR), O121 amp C, O121 amp P strep C, O157:H7 H1730 amp C and O157:H7 H1730 amp P strep C were not inactivated after exposure to 1% and 2.5% v/v lactic acid for 300 s. No recovery of cells was observed after the strains were exposed to 5% v/v lactic acid for 300 s. ABR strains O157:H7 H1730 amp C and O157: H7 H1730 amp P strep C demonstrated a high tolerance to lactic acid (P ≤ 0.05).

Conclusion: ABR in isolate E. coli O157: H7 H1730 may improve tolerance to lactic acid. Increased tolerance may be discerned by evaluating growth parameters of bacteria in presence of sub-MIC levels of lactic acid.

Keywords: MIC; Shiga-toxin producing E. coli; antibiotic resistance; cross-tolerance; lactic acid.