Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer

Feng Wang; Xiaolei Yu; Zhuo Yu; Yi Cui; Ling Xu; Shuhao Huo; Zhongyang Ding; Liting Zhao; Lizhi Du; Yanguo Qiu

doi:10.3389/fbioe.2023.1176352

Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer

Front Bioeng Biotechnol. 2023 Apr 25:11:1176352. doi: 10.3389/fbioe.2023.1176352. eCollection 2023.

Authors

Feng Wang^{1

2}, Xiaolei Yu¹, Zhuo Yu¹, Yi Cui¹, Ling Xu^{1

2}, Shuhao Huo¹, Zhongyang Ding³, Liting Zhao³, Lizhi Du⁴, Yanguo Qiu⁴

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
² Institute of Agricultural Products Processing Engineering, Jiangsu University, Zhenjiang, China.
³ Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
⁴ Shandong Dehemingxing Biotechnology Co., Ltd., Weifang, China.

Abstract

A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor. After medium optimization, laccase activity increased by 12.77-fold compared to that without GHK-Cu. The laccase production of 1113.8 U L^-1 was obtained by scaling-up culture in 5-L stirring tank. The laccase production induced by CuSO₄ was poorer than that of GHK-Cu at the same mole concentration. GHK-Cu could increase the permeability of cell membrane with less damage, and it facilitated the adsorption, accumulation, and utilization of copper by fungal cells, which was beneficial for laccase synthesis. GHK-Cu induced better expression of laccase related genes than that of CuSO₄, resulting in higher laccase production. This study provided a useful method for induced production of laccase by applying GHK chelated metal ion as a non-toxic inducer, which reduced the safety risk of laccase broth and provided the potential application of crude laccase in food industry. In addition, GHK can be used as the carrier of different metal ions to enhance the production of other metalloenzymes.

Keywords: Trametes versicolor; copper-Glycyl-L-Histidyl-L-Lysine; induction; laccase; medium optimization.

Grants and funding

This work was supported by the National Key Research and Development Program of China, grant number 2020YFA0907300; the National Natural Science Foundation of China, grant number No. 21978120; the Key R&D project in Jiangsu Province, grant number BE2020405 and the Senior Talent Scientific Research Initial Funding Project of Jiangsu University, grant numbers 15JDG17 and 15JDG061.