Low-Density Lipoprotein Receptor LRP-2 regulates GLR-1 glutamate receptors and glutamatergic behavior in C. elegans

Bethany J Rennich; Eric S Luth; Julia Hofer; Peter Juo

doi:10.17912/micropub.biology.000837

Low-Density Lipoprotein Receptor LRP-2 regulates GLR-1 glutamate receptors and glutamatergic behavior in C. elegans

MicroPubl Biol. 2023 Apr 26:2023:10.17912/micropub.biology.000837. doi: 10.17912/micropub.biology.000837. eCollection 2023.

Authors

Bethany J Rennich^{1

2}, Eric S Luth^{3

2}, Julia Hofer², Peter Juo^{1

2}

Affiliations

¹ Program in Neuroscience, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111.
² Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111.
³ Biology, Simmons University, Boston, MA 02115.

Abstract

We identified the Low-Density Lipoprotein (LDL) Receptor Related Protein-2 (LRP-2) in a RNAi screen for genes that regulate glutamatergic behavior in C. elegans . lrp-2 loss-of-function mutants have defects in glutamatergic mechanosensory nose-touch behavior and suppress increased spontaneous reversals induced by GLR-1(A/T), a constitutively-active form of the AMPA-type glutamate receptor GLR-1. Total and surface levels of GLR-1 are increased throughout the ventral nerve cord of lrp-2 mutants suggesting that LRP-2 promotes glutamatergic signaling by regulating some aspect of GLR-1 trafficking, localization or function.

Grants and funding

The Caenorhabditis Genetics Center is funded by the NIH Office of Research Infrastructure Programs P40 OD010440. This work was supported in part by a National Science Foundation Grant IOS#1941073 (to P.J.), a National Institutes of Health grant R21NS101534 (to P.J.), a National Institutes of Health predoctoral fellowship grant #F31NS120586 (to B.J.R.) and a National Institutes of Health IRACDA grant #5K12GM074869 (to E.S.L.).