Detection of Mannheimia haemolytica-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle

Korakrit Poonsuk; Carita Kordik; Matthew Hille; Ting-Yu Cheng; William B Crosby; Amelia R Woolums; Michael L Clawson; Carol Chitko-McKown; Bruce Brodersen; John Dustin Loy

doi:10.3390/ani13091531

Detection of Mannheimia haemolytica-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle

Animals (Basel). 2023 May 3;13(9):1531. doi: 10.3390/ani13091531.

Affiliations

¹ Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA.
² Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
³ Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
⁴ United States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USA.

Abstract

Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of M. haemolytica (p-value < 0.05). These data suggest that M. haemolytica ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.

Keywords: ELISA; IgA; IgG; IgM; Mannheimia haemolytica; antibody; bovine respiratory disease.

Grants and funding

This research was partially funded by the Nebraska Experiment Station with funds from the Animal Health and Disease Research (section 1433) and Hatch capacity funding programs (accessions# 1002196: 1007070 and 1017646) through the USDA National Institute of Food and Agriculture. Additionally funding was provided by the Nebraska Veterinary Diagnostic Center. The use of product and company names is necessary to accurately report the methods and results; however, the United States Department of Agriculture (USDA) neither guarantees nor warrants the standard of the products, and the use of names by the USDA implies no approval of the product to the exclusion of others that may also be suitable.