Enzyme Cascade Reactions on DNA Origami Scaffold

Eiji Nakata; Huyen Dinh; Peng Lin; Takashi Morii

doi:10.1007/978-1-0716-3028-0_16

Enzyme Cascade Reactions on DNA Origami Scaffold

Methods Mol Biol. 2023:2639:275-299. doi: 10.1007/978-1-0716-3028-0_16.

Authors

Eiji Nakata¹, Huyen Dinh¹, Peng Lin¹, Takashi Morii²

Affiliations

¹ Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
² Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan. t-morii@iae.kyoto-u.ac.jp.

PMID: 37166723
DOI: 10.1007/978-1-0716-3028-0_16

Abstract

The protocols for constructing, characterizing, and analyzing enzyme cascade reaction systems on the DNA scaffold are described. Two-step and three-step enzyme cascade reactions were adapted from the xylose metabolic pathway as the example of natural metabolic pathway and were assembled on the DNA scaffold by using the DNA binding adaptors.

Keywords: AFM; Assembling yield; DNA binding adaptor; DNA origami; DNA scaffold; Enzyme cascade reaction; Volume analysis; Xylose metabolic pathway.

MeSH terms

DNA* / chemistry
Nanostructures* / chemistry
Nucleic Acid Conformation

Substances

DNA