Development of sensitive and stable electrochemical impedimetric biosensor based on T1R1 receptor and its application to detection of umami substances

Yijian Wang; Liqin Kong; Guoqiang Shu; Guanqquan Sun; Yaoze Feng; Ming Zhu

doi:10.1016/j.foodchem.2023.136233

Development of sensitive and stable electrochemical impedimetric biosensor based on T1R1 receptor and its application to detection of umami substances

Food Chem. 2023 Oct 15:423:136233. doi: 10.1016/j.foodchem.2023.136233. Epub 2023 Apr 23.

Authors

Yijian Wang¹, Liqin Kong², Guoqiang Shu², Guanqquan Sun², Yaoze Feng³, Ming Zhu²

Affiliations

¹ College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen 518000, PR China; Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China.
² College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China; Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China.
³ College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen 518000, PR China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, PR China; Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China. Electronic address: yaoze.feng@mail.hzau.edu.cn.

PMID: 37156142
DOI: 10.1016/j.foodchem.2023.136233

Abstract

Umami substances can provide a palatable flavour for food. In this study, an electrochemical impedimetric biosensor was developed for detecting umami substances. This biosensor was fabricated by immobilising T1R1 onto AuNPs/reduced graphene oxide/chitosan which was in advance electro-deposited onto a glassy carbon electrode. The evaluation by the electrochemical impedance spectrum method showed that the T1R1 biosensor performed well with low detection limits and wide linear ranges. Under the optimised incubation time (60 s), the electrochemical response was linearly related to the concentrations of the detected targets monosodium glutamate and inosine-5'-monophosphate within their respective linear range of 10^-14 to 10^-9 M and 10^-16 to 10^-13 M. The low detection limit of monosodium glutamate and inosine-5'-monophosphate was 10^-15 M and 10^-16 M, respectively. Moreover, the T1R1 biosensor exhibited high specificity to umami substances even in the real food sample. The developed biosensor still retained 89.24% signal intensity after 6-day storage, exhibiting a desirable storability.

Keywords: Electrochemical biosensor; Electrochemical impedance spectroscopy; T1R1 receptor; Umami.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Gold
Inosine
Inosine Monophosphate
Metal Nanoparticles*
Receptors, G-Protein-Coupled / chemistry
Sodium Glutamate

Substances

Sodium Glutamate
Receptors, G-Protein-Coupled
Gold
Inosine Monophosphate
Inosine