Longan pulp polysaccharides regulate gut microbiota and metabolites to protect intestinal epithelial barrier

Yajuan Bai; Yue Zhou; Xiang Li; Ruifen Zhang; Fei Huang; Bei Fan; Litao Tong; Fengzhong Wang; Mingwei Zhang

doi:10.1016/j.foodchem.2023.136225

Longan pulp polysaccharides regulate gut microbiota and metabolites to protect intestinal epithelial barrier

Food Chem. 2023 Oct 1:422:136225. doi: 10.1016/j.foodchem.2023.136225. Epub 2023 Apr 27.

Authors

Yajuan Bai¹, Yue Zhou², Xiang Li¹, Ruifen Zhang³, Fei Huang³, Bei Fan¹, Litao Tong⁴, Fengzhong Wang⁵, Mingwei Zhang⁶

Affiliations

¹ Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Sanya Institute, Hainan Academy of Agricultural Sciences, Haikou 572025, PR China.
² Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission of China/College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China.
³ Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
⁴ Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
⁵ Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Sanya Institute, Hainan Academy of Agricultural Sciences, Haikou 572025, PR China. Electronic address: wangfengzhong@sina.com.
⁶ Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China. Electronic address: mwzhh@vip.tom.com.

PMID: 37156018
DOI: 10.1016/j.foodchem.2023.136225

Abstract

Longan pulp polysaccharide is a bioactive component with prebiotic activity and intestinal barrier protection. This study aimed to evaluate the influence of digestion and fermentation on the bioavailability and intestinal barrier protection of polysaccharide LPIIa from longan pulp. The molecular weight of LPIIa didn't change significantly after gastrointestinal digestion in vitro. After fecal fermentation, 56.02% of LPIIa was consumed by gut microbiota. The short-chain fatty acid level in LPIIa group was 51.63% higher than that in blank group. LPIIa intake also increased short-chain fatty acid production and G-protein-coupled receptor 41 expression in the colon of mice. Moreover, LPIIa improved the relative richness of Lactobacillus, Pediococcus, and Bifidobacterium in colon content. Compared to LPIIa, fecal fermented LPIIa better protected intestinal epithelial barrier by increasing Zonula occludens-1 expression. These results provided an important basis for the design of functional food based on longan polysaccharides to prevent intestinal barrier damage related diseases.

Keywords: Bioavailability; Digestion and fermentation; Intestinal epithelial barrier; Longan pulp polysaccharide; Prebiotic effect.

MeSH terms

Animals
Digestion
Fatty Acids, Volatile / metabolism
Feces / microbiology
Fermentation
Gastrointestinal Microbiome* / physiology
Mice
Polysaccharides / metabolism
Polysaccharides / pharmacology

Substances

Polysaccharides
Fatty Acids, Volatile

Supplementary concepts

Dimocarpus