New probiotics (Lactobacillus plantarum and Saccharomyces cerevisiae) supplemented to fermented rice straw-based rations on digestibility and rumen characteristics in vitro

Yetti Marlida; Harnentis Harnentis; Yuliaty Shafan Nur; Laily Rinda Ardani

doi:10.5455/javar.2023.j657

New probiotics (Lactobacillus plantarum and Saccharomyces cerevisiae) supplemented to fermented rice straw-based rations on digestibility and rumen characteristics in vitro

J Adv Vet Anim Res. 2023 Mar 31;10(1):96-102. doi: 10.5455/javar.2023.j657. eCollection 2023 Mar.

Authors

Yetti Marlida¹, Harnentis Harnentis¹, Yuliaty Shafan Nur¹, Laily Rinda Ardani²

Affiliations

¹ Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang, Indonesia.
² Ph.D. Student (PMDSU Program), Graduate Program Faculty of Animal Science, Andalas University, Padang, Indonesia.

Abstract

Objective: This research was arranged to explore the effect of supplementation of a combination of Lactobacillus plantarum and Saccharomyces cerevisiae as a new probiotic in fermented rice straw-based rations on in vitro digestibility and ruminal characteristics.

Materials and methods: A randomized group design with 3 types of treatment and 4 replications as a group was used in this study. A probiotic inoculum containing L. plantarum and S. cerevisiae with 1 × 10¹⁰ colony-forming unit (CFU)/ml. Treatments were followed by: P1 = complete rations without probiotics (control), P2 = P1 supplemented 0.5% probiotics, and P3 = P1 supplemented 1% probiotics. Substrate complete rations were based on the fermented rice straw and concentrate (60%:40%). Parameters of digestibility and rumen fermentation products were determined after 48 h of incubation.

Results: Probiotics supplemented with fermented rice straw-based rations significantly increased (p < 0.05) digestibility and rumen characteristics in vitro. Supplementation with 1% probiotics (P3) produces the highest digestibility compared to other treatments: in-vitro dry matter digestibility (IVDMD) (55%), in-vitro organic matter digestibility (IVOMD) (58.28%), in-vitro crude protein digestibility (IVCPD) (84.42%), in-vitro acid detergent fiber digestibility (IVADFD) (53.99%), in-vitro neutral detergent fiber digestibility (IVNDFD) (58.39%), and in-vitro cellulose digestibility (IVCLD) (67.12%). Rumen pH (6.76-6.80) did not change significantly (p > 0.05) due to supplemented probiotics. Probiotic supplementation in rations significantly (p < 0.05) increased the content of NH₃ and total volatile fatty acid (VFA). Supplementation with 1% probiotic (P3) showed the highest concentration of NH₃ (26.56 mg/100 ml) and was also followed by the total VFA (115.75 mM) compared to the control (22.59 mg/100 ml and 103.00 mM, respectively).

Conclusion: Supplementation of 1% probiotics (combination of L. plantarum and S. cerevisiae) containing 1 × 10¹⁰ CFU/ml in fermented rice straw-based rations increases nutrient digestibility, that is, IVDMD, IVOMD, IVCPD, IVADFD, IVNDFD, and IVCLD, and also increases rumen fermentation, which is the concentration of NH₃ and total VFA.

Keywords: Digestibility; lactic acid bacteria; probiotics; rice straw; rumen characteristics; yeast.