First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

Md Muket Mahmud; Ajran Kabir; Md Zawad Hossain; Sanjida Jamal Mim; Israt Jahan Yeva; Minara Khatun; Mohammad Saidur Rahman; Madan Mohan Dey; K H M Nazmul Hussain Nazir

doi:10.5455/javar.2023.j659

First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

J Adv Vet Anim Res. 2023 Mar 31;10(1):113-117. doi: 10.5455/javar.2023.j659. eCollection 2023 Mar.

Authors

Affiliations

¹ Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
² These two authors contributed equally.
³ Department of Agricultural Economics, Bangladesh Agricultural University, Mymensingh, Bangladesh.
⁴ Department of Agricultural Sciences, Texas State University, San Marcos, TX, USA.

Abstract

Objective: This study aimed to isolate Aliarcobacter cryaerophilus in ready-to-cook poultry meat in Bangladesh.

Materials and methods: Thirty drumstick samples were collected from super shops in Dhaka city (n = 10), Mymensingh city (n = 10), and Patuakhali town (n = 10). After sample processing, they were cultured in Blood agar media with Campylobacter base using a microfilter (0.42 nm). Suspected colonies were subjected to DNA extraction and PCR assay targeting 16SrRNA genes. Then, sequencing was performed for confirmation.

Results: Of 30 samples, 3 (10%) were positive for A. cryaerophilus. Phylogenetic analysis shows that our isolate has strong similarities with one of the isolates from China.

Conclusion: The presence of this organism in ready-to-cook poultry meat is a significant concern for consumers as it bears zoonotic importance.

Keywords: 16SrRNA; Aliarcobacter cryaerophilus; PCR; phylogenetic analysis; poultry meat; super shops.