Trichoscopic evaluation of dental pulp stem cell conditioned media for androgenic alopecia

Tomoko Kamishima; Chie Hirabe; Takanori Ohnishi; Junichi Taguchi; Khin Zay Yar Myint; Shoji Koga

doi:10.1111/jocd.15799

Trichoscopic evaluation of dental pulp stem cell conditioned media for androgenic alopecia

J Cosmet Dermatol. 2023 Nov;22(11):3107-3117. doi: 10.1111/jocd.15799. Epub 2023 May 8.

Authors

Tomoko Kamishima¹, Chie Hirabe¹, Takanori Ohnishi², Junichi Taguchi³, Khin Zay Yar Myint⁴, Shoji Koga^{5

6}

Affiliations

¹ Department of Dermatology, Tokyo Midtown Skin/Aesthetic Clinic Noage, Tokyo, Japan.
² Tokyo Midtown Dental Clinic, Tokyo, Japan.
³ Tokyo Midtown Clinic, Tokyo, Japan.
⁴ Tokyo Midtown Center for Advanced Medical Science and Technology, Tokyo, Japan.
⁵ Ginza Solaria Clinic, Tokyo, Japan.
⁶ Panagy Co., Ltd., Tokyo, Japan.

PMID: 37154468
DOI: 10.1111/jocd.15799

Abstract

Background: Conditioned media (CM) derived from mesenchymal stem cells (MSC) is known to induce hair regrowth in androgenic alopecia.

Objectives: The objectives of the study were to assess the efficacy and safety of one type of MSC-CM, the CM derived from dental pulp stem cells obtained from human exfoliated deciduous teeth (SHED-CM) and to compare the efficacy of SHED-CM with and without dihydrotestosterone synthesis inhibitor (DHT-inhibitor).

Methods: Eighty-eight male androgenic alopecia subjects with Hamilton-Norwood Classification (H-N C) I-VII were evaluated by trichoscopy to explore which trichoscopic factors statistically correlated with H-N C. After being screened, 33 subjects received six SHED-CM treatments at 1-month intervals. Clinical severity was assessed through global and trichoscopic images from baseline to 9th month.

Results: SHED-CM was effective for 75% of subjects regardless of disease severity, concomitant DHT-inhibitor use, and age. Adverse effects including pain and small hemorrhages were transient and mild. We also found that clinical hair status evaluated by absolute values of three quantitative trichoscopic factors (maximum hair diameter, vellus hair rate, and multi-hair follicular unit rate) showed a good correlation with H-N C stages, and what is more-a scoring system of these three factors can be a possible predictor of SHED-CM efficacy.

Conclusions: We have shown that SHED-CM provides global and trichoscopic image improvement for androgenic alopecia, regardless of concomitant DHT-inhibitor use.

Keywords: androgenic alopecia; conditioned media; dental pulp stem cell; hair loss; trichoscopy.