Profiling of chemical constituents of Matricarla chamomilla L. by UHPLC-Q-Orbitrap-HRMS and in vivo evaluation its anti-asthmatic activity

Qian Li; Rahima Abdulla; Xuelei Xin; Guipeng Xue; Xiaolong Kang; Feicui Zhao; Haji Akber Asia

doi:10.1016/j.heliyon.2023.e15470

Profiling of chemical constituents of Matricarla chamomilla L. by UHPLC-Q-Orbitrap-HRMS and in vivo evaluation its anti-asthmatic activity

Heliyon. 2023 Apr 24;9(5):e15470. doi: 10.1016/j.heliyon.2023.e15470. eCollection 2023 May.

Authors

Qian Li^{1

2

3}, Rahima Abdulla³, Xuelei Xin³, Guipeng Xue⁴, Xiaolong Kang^{1

2}, Feicui Zhao^{1

2}, Haji Akber Asia³

Affiliations

¹ Department of Medicine Research, Hospital of Chinese Medicine Affiliated to Xinjiang Medical University, Urumqi, 830000, People's Republic of China.
² Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine, Urumqi, 830000, People's Republic of China.
³ CAS Key Laboratory of Chemistry of Plant Resources in Arid Regions, Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1, Beijing Road, Urumqi, 830011, Xinjiang, China.
⁴ Xinjiang Uygur Autonomous Region Evaluation and Inspection Center for Drug, Urumqi, 830000, People's Republic of China.

Abstract

Matricarla chamomilla L. is native to European countries and widely cultivated in China, especially in Xinjiang. It has been used in Uygur medicine for the treatment of cough caused by asthma. In this study, UHPLC-Q-Orbitrap-MS was used to detect and identify the components from the active fraction of M. Chamomile, 64 compounds were identified by combining the standards, related literatures and mass spectrometry fragments, including 10 caffeoyl quinic acids, 38 flavonoids, 8 coumarins, 5 alkaloids and 3 other compounds. Furtherly, the anti-asthma activity of active fraction of M. Chamomile was investigated in OVA-induced allergic asthma rat model. The results showed that the number of EOS in Penh and bronchoalveolar lavage fluid (BALF) in the group of the active fraction of M. Chamomile was significantly lower than that in the model group. Besides, the active fraction of M. Chamomile can significantly reduce the IgE level and increased glutathione peroxidase (GSH-Px) in the serum of OVA-induced rats, and ameliorated OVA-induced lung injury. Hence, M. Chamomile could be used to treat asthma through their in vivo antioxidant and anti-inflammatory effects. This study explored the potential material basis of M. Chamomile for the treatment of asthma.

Keywords: Anti-asthmatic activity; Mass; Matricarla chamomilla L.; UHPLC.