Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Katerina Vlahos; Koula Sourris; Jia Yi Kuah; Alison Graham; Annabelle Suter; Sara E Howden; Edouard G Stanley; Andrew G Elefanty

doi:10.1016/j.scr.2023.103109

Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Stem Cell Res. 2023 Jun:69:103109. doi: 10.1016/j.scr.2023.103109. Epub 2023 May 3.

Authors

Katerina Vlahos¹, Koula Sourris², Jia Yi Kuah², Alison Graham², Annabelle Suter², Sara E Howden³, Edouard G Stanley⁴, Andrew G Elefanty⁴

Affiliations

¹ Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia. Electronic address: Katerina.Vlahos@mcri.edu.au.
² Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia.
³ Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Parkville, VIC, Australia.
⁴ Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Parkville, VIC, Australia.

PMID: 37150143
DOI: 10.1016/j.scr.2023.103109

Abstract

We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells* / metabolism
Kruppel-Like Factor 4
Leukocytes, Mononuclear / metabolism

Substances

Kruppel-Like Factor 4