A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses

Tugeqin Bou; Wenqi Ding; Huiying Liu; Wendian Gong; Zijie Jia; Manglai Dugarjaviin; Dongyi Bai

doi:10.1016/j.cbd.2023.101084

A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Jun:46:101084. doi: 10.1016/j.cbd.2023.101084. Epub 2023 May 2.

Authors

Tugeqin Bou¹, Wenqi Ding², Huiying Liu², Wendian Gong², Zijie Jia², Manglai Dugarjaviin², Dongyi Bai³

Affiliations

¹ Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: https://twitter.com/@BTvgqin.
² Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
³ Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: baidongyi1983@163.com.

PMID: 37150091
DOI: 10.1016/j.cbd.2023.101084

Abstract

The proportion of different muscle fibers is essential for the horse breed's aptitude for athletic activities. Adaptation of locomotor muscle is correlated with altered physiologic conditions. To investigate the adaptive changes of muscle fiber phenotype and transcriptome in horse skeletal muscle during dietary restriction (DR). The muscle fiber type distribution and deep RNA-seq analysis of detecting differentially expressed mRNAs (DEGs), miRNA (DEMIRs), lncRNAs (DELs), circRNAs (DECs), and their function analysis were investigated in gluteus medius muscle of Mongolian horses during DR. A total of 1433 DEGs, 5 DEMIRs, 329 DELs, and 53 DECs were identified. Differing from non-uniform muscle fiber type changing, functional enrichment analysis showed that most downregulated DEGs were associated in muscle contraction, fuel energy metabolism, and protein balance. Linkages between non-coding RNA and mRNA landscape were detected from their functional changes. Our study provides new insights into the expressional changes of mRNA and non-coding RNA in horse skeletal muscles during DR, which might improve our understanding of the molecular mechanisms regulating muscle adaption during DR for racing horses.

Keywords: Dietary restriction; Expression profile; Non-coding RNA; Skeletal muscle; Transcriptomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Expression Profiling
Horses / genetics
MicroRNAs* / genetics
Muscle, Skeletal / metabolism
RNA, Circular / genetics
RNA, Long Noncoding* / genetics
RNA, Messenger / genetics

Substances

RNA, Long Noncoding
MicroRNAs
RNA, Circular
RNA, Messenger