Biocompatibility of magnetic nanoparticles synthesized through green routed with a focus on hematological and histological analysis

Zhaleh Fakhri; Nasser Karimi; Fakhredin Saba; Mohesn Zhaleh

doi:10.1016/j.bioorg.2023.106552

Biocompatibility of magnetic nanoparticles synthesized through green routed with a focus on hematological and histological analysis

Bioorg Chem. 2023 Aug:137:106552. doi: 10.1016/j.bioorg.2023.106552. Epub 2023 Apr 20.

Authors

Zhaleh Fakhri¹, Nasser Karimi², Fakhredin Saba³, Mohesn Zhaleh³

Affiliations

¹ Nanobiotechnology Department, Faculty of Innovative Science and Technology, Razi University, Kermanshah, Iran.
² Nanobiotechnology Department, Faculty of Innovative Science and Technology, Razi University, Kermanshah, Iran; Laboratory of Plant Physiology, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran. Electronic address: nkarimi@razi.ac.ir.
³ Department of Medical Laboratory Science, School of Paramedical, Kermanshah University of Medical Sciences, Kermanshah, Iran.

PMID: 37149950
DOI: 10.1016/j.bioorg.2023.106552

Abstract

The interaction of metal nanoparticles (MNPs) with blood cells and tissues is essential from the perspectives of biocompatibility and the production of novel drug delivery systems. In the present study, biosynthesized-Fe₃O₄ nanoparticles (bio-Fe₃O₄ NPs) were prepared and bio-modified using Daphne mucronata Royle leaf extracts. The physicochemical properties of bio-Fe₃O₄ NPs were determined using UV-Visible spectroscopy, Energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), dynamic light scattering (DLS), and Fourier transform infrared (FT-IR) analyses. According to the SEM analysis, the bio-Fe₃O₄ NPs are spherical-shaped with a size range of 10-30 nm. Antibacterial effects of bio-Fe₃O₄ NPs against Staphylococcus aureus ATCC 43300 and Pseudomonas aeruginosa ATCC 27853 bacteria were measured by minimum inhibition/bactericidal concentrations (MIC and MBC tests). Result showed that the bio-Fe₃O₄ NPs (300 ppm) revealed highest antibacterial effect on S. aureus ATCC 43300. Also, bio-Fe₃O₄ NPs have different cell viability in the human breast cancer cell line (MCF-7) and mouse embryonic fibroblast (MEF). The interaction of bio-Fe₃O₄ NPs with blood cells and the complete blood count (CBC) factor illustrated that the morphology of blood cells and platelet clumping did not influence by nanoparticles. Furthermore, histological analysis of the liver, spleen, and kidney did not show any abnormality upon exposure to 100 mg kg^-1 bio-Fe₃O₄ NPs treated samples. Hence, the biosynthesized Fe₃O₄ NPs are a good candidate for applications in medical fields.

Keywords: Antibacterial activities; Bio-Fe(3)O(4) NPs; Complete blood count; MCF-7 cells line; MEF cells line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Fibroblasts
Humans
Magnetite Nanoparticles* / chemistry
Mice
Microbial Sensitivity Tests
Plant Extracts / chemistry
Plant Extracts / pharmacology
Spectroscopy, Fourier Transform Infrared
Staphylococcus aureus*

Substances

Magnetite Nanoparticles
Anti-Bacterial Agents
Plant Extracts