Synergistic antibacterial effect of multifunctional TiO2-X-based nanoplatform loading arginine and polydopamine for promoting infected wounds healing

Shuqing Xiang; Mingqian Wang; Li Li; Jian Shen

doi:10.1016/j.colsurfb.2023.113332

Synergistic antibacterial effect of multifunctional TiO_2-X-based nanoplatform loading arginine and polydopamine for promoting infected wounds healing

Colloids Surf B Biointerfaces. 2023 Jun:226:113332. doi: 10.1016/j.colsurfb.2023.113332. Epub 2023 May 3.

Authors

Shuqing Xiang¹, Mingqian Wang², Li Li³, Jian Shen⁴

Affiliations

¹ National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China.
² National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China. Electronic address: wmqnju@126.com.
³ National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China. Electronic address: lili3@njnu.edu.cn.
⁴ National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China; Jiangsu Engineering Research Center of Interfacial Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Qixia District, Nanjing 210023, China. Electronic address: jshen@njnu.edu.cn.

PMID: 37148663
DOI: 10.1016/j.colsurfb.2023.113332

Abstract

The gas therapy of some endogenous signaling molecules to treat diseases has caused extensive research, among which NO gas has shown great potential in fighting infection with various pathogens, promoting wound healing, etc. Here, we propose a photothermal/photodynamic/NO synergistic antibacterial nanoplatform by loading L-arginine (LA) on mesoporous TiO₂ and then encapsulating it with polydopamine. The obtained TiO_2-x-LA@PDA nanocomposite possesses both the excellent photothermal effect and ROS generation ability of mesoporous TiO₂, and the release of nitric oxide (NO) from L-arginine under near-infrared (NIR) light irradiation, while the sealing layer of PDA could induce NIR-triggered NO controlled release. In vitro antibacterial experiments confirmed that the synergistic effect of TiO_2-x-LA@PDA nanocomposites has excellent antibacterial effects against Gram-negative and Gram-positive bacteria, while in vivo experiments showed that it has lower toxicity. It is worth noting that compared with the pure photothermal effect and ROS, the generated NO showed a better bactericidal effect, and NO had a better ability to promote wound healing. In conclusion, the developed TiO_2-x-LA@PDA nanoplatform can be used as a nanoantibacterial agent, which can be further explored in the related biomedical field of photothermal activation of multimodal combined antibacterial therapy.

Keywords: Black mesoporous titanium dioxide; Gas therapy; Photodynamic therapy; Photothermal therapy; Synergistic antibacterial.

MeSH terms

Anti-Bacterial Agents* / pharmacology
Reactive Oxygen Species
Wound Healing*

Substances

titanium dioxide
polydopamine
Reactive Oxygen Species
Anti-Bacterial Agents